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The single antigen expressing lines (SALs) concept: an excellent tool for screening for HLA-specific antibodies.
- Source :
-
Human immunology [Hum Immunol] 2005 May; Vol. 66 (5), pp. 519-25. Date of Electronic Publication: 2005 Feb 12. - Publication Year :
- 2005
-
Abstract
- Definition of the antibody specificity in the serum of patients waiting for a renal transplant or in need for platelet transfusion is a crucial step for finding adequate donors. Confounding factors are the complexity of the serum antibodies and the expression of several, up to six, different human leukocyte antigens (HLA) on peripheral blood lymphocytes used as target cells in the antibody screening. Single antigen-expressing (SAL) cell lines were generated by transfecting human major histocompatibility complex (MHC) class I sequences into K562, an erythroleukemia-derived cell line lacking MHC class I and II expression. Thirty-seven different SALs have been generated so far. In this study, we present the validation of 16 of those SALs by flow cytometry against a panel of 84 human HLA-specific monoclonal antibodies (30 HLA-A [8 IgG/22 IgM], 45 HLA-B [18 IgG/27 IgM], 6 HLA-A, B [3 IgG/3 IgM], and 3 HLA-C [all IgM]) developed in our laboratory. The SALs proved to be suitable tools to determine acceptable mismatches for highly sensitized patients. This concept of transfecting target sequences in immortalized cell lines opens up new avenues in the definition of serum and cellular reactivity for sensitized patients awaiting a suitable organ or blood component.
- Subjects :
- Antibodies blood
Antibodies, Monoclonal immunology
Antibody Affinity immunology
Antibody Specificity immunology
Cell Line, Tumor
Complement System Proteins immunology
Cross Reactions immunology
Cytotoxicity Tests, Immunologic
Female
Flow Cytometry
HLA Antigens genetics
HLA Antigens metabolism
HLA-A2 Antigen genetics
HLA-A2 Antigen immunology
HLA-A3 Antigen genetics
HLA-A3 Antigen immunology
HLA-B7 Antigen genetics
HLA-B7 Antigen immunology
Histocompatibility Antigens Class I genetics
Histocompatibility Antigens Class I immunology
Histocompatibility Antigens Class I metabolism
Humans
Immunoassay methods
Immunoglobulin G immunology
Immunoglobulin M immunology
K562 Cells
Leukocytes, Mononuclear immunology
Transfection
Antibodies immunology
HLA Antigens immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0198-8859
- Volume :
- 66
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Human immunology
- Publication Type :
- Academic Journal
- Accession number :
- 15935889
- Full Text :
- https://doi.org/10.1016/j.humimm.2005.01.007