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[Establishment of COL1A1-EGFP stably transfected ROS17/2.8 cell lines].
- Source :
-
Hang tian yi xue yu yi xue gong cheng = Space medicine & medical engineering [Space Med Med Eng (Beijing)] 2004 Apr; Vol. 17 (2), pp. 107-10. - Publication Year :
- 2004
-
Abstract
- Objective: To obtain ROS17/2.8 cell lines which were stably expressing EGFP reporter gene drived by COL1A1 promoter.<br />Method: A 3.6 Kb COL1A1 promoter from rat was cloned into pMD-18-T vector by PCR. This amplified promoter vector was digested to get several different length fragments which were then fused with EGFP reporter gene to construct eukaryotic expression vectors. ROS17/2.8 cell was stably transfected with these vectors by LipofectAMINE(TM) and selected by G418.<br />Result: The COL1A1-EGFP stably transfected cell lines were established.<br />Conclusion: The cell lines will be useful for studying the effects of microgravity on the activity of COL1A1 promoter and expression of gene related with bone form.
- Subjects :
- Alkaline Phosphatase genetics
Animals
Cell Line
Collagen Type I, alpha 1 Chain
Genetic Vectors
Immunohistochemistry
Osteoblasts
Osteosarcoma genetics
Polymerase Chain Reaction
Rats
Weightlessness Simulation
Collagen Type I genetics
Gene Expression Regulation
Green Fluorescent Proteins genetics
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1002-0837
- Volume :
- 17
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Hang tian yi xue yu yi xue gong cheng = Space medicine & medical engineering
- Publication Type :
- Academic Journal
- Accession number :
- 15909385