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Detection of Cryptosporidium parvum oocysts on fresh vegetables and 1 herbs using antibodies specific for a Cryptosporidium parvum viral antigen.
- Source :
-
Journal of food protection [J Food Prot] 2005 May; Vol. 68 (5), pp. 1093-6. - Publication Year :
- 2005
-
Abstract
- The purpose of this study was to determine if the viral symbiont of Cryptosporidium parvum (CPV) sporozoites could be used as a target for sensitive detection of the parasite in food samples. Polyclonal sera specific to the recombinant viral capsid protein (rCPV40) was used in a dot blot hybridization assay to detect oocysts recovered from green onions and cilantro. Small batches of chopped green onions and cilantro leaves were artificially contaminated with three different concentrations of oocysts: 10(6), 10(2), and 10(1). rCPV40 was superior in detecting oocysts compared with other antibodies directed toward total oocyst protein and oocyst surface antigens. This study provides evidence that CPV is an excellent target for sensitive detection of C. parvum oocysts in foods.
- Subjects :
- Animals
Capsid Proteins genetics
Capsid Proteins immunology
Food Contamination analysis
Oocysts virology
Parasite Egg Count
Sensitivity and Specificity
Antigens, Viral genetics
Coriandrum parasitology
Cryptosporidium parvum isolation & purification
Cryptosporidium parvum virology
Food Parasitology
Onions parasitology
Oocysts isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 0362-028X
- Volume :
- 68
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of food protection
- Publication Type :
- Academic Journal
- Accession number :
- 15895749
- Full Text :
- https://doi.org/10.4315/0362-028x-68.5.1093