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Induction of oxidative stress and antioxidative mechanisms in Phaseolus vulgaris after Cd application.
- Source :
-
Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2005 May; Vol. 43 (5), pp. 437-44. Date of Electronic Publication: 2005 Apr 25. - Publication Year :
- 2005
-
Abstract
- Oxidative stress has been shown to be of great importance in the toxicity of several metals (copper, zinc, ...). In this study, the relationship of cadmium phytotoxicity and antioxidative reactions in bean (Phaseolus vulgaris L.) plants was investigated. Eleven-day-old seedlings were exposed to an environmentally realistic concentration of cadmium (2 microM CdSO(4)). Several biochemical and physiological parameters were influenced even by these low concentrations. At the biochemical level, the antioxidative defence mechanism was significantly activated after 24 h of cadmium exposure. Some enzymes able of quenching reactive oxygen species (syringaldazine peroxidase, EC 1.11.1.7; guaiacol peroxidase, EC 1.11.1.7) as well as enzymes important in the reduction of NAD(P)(+) (isocitrate dehydrogenase, EC 1.1.1.42; malic enzyme, EC 1.1.1.40) were significantly elevated by cadmium exposure. Furthermore, the ascorbate-glutathione cycle appeared to be a very important mechanism against cadmium-induced oxidative stress. In leaves, significant increases of ascorbate peroxidase (EC 1.11.1.11) and glutathione reductase (EC 1.6.4.2) and significant changes in the ascorbate and glutathione pool were observed. Morphological and other biochemical parameters (lipid peroxidation) were significantly enhanced 48 h after the start of the cadmium exposure. At the end of the experiment (72 h after the start of the metal treatment), even visual effects, such as chlorosis, were observed. The present data indicate that cadmium, like other metals, induces cellular redox disequilibrium suggesting that an environmentally realistic concentration of cadmium can cause oxidative stress.
- Subjects :
- Antioxidants metabolism
Ascorbate Peroxidases
Cadmium metabolism
Glutathione metabolism
Glutathione Disulfide metabolism
Glutathione Reductase metabolism
Isocitrate Dehydrogenase metabolism
Lipid Peroxidation drug effects
Malate Dehydrogenase metabolism
Peroxidase metabolism
Peroxidases metabolism
Phaseolus drug effects
Phaseolus growth & development
Plant Leaves drug effects
Plant Leaves growth & development
Plant Leaves metabolism
Reactive Oxygen Species metabolism
Time Factors
Cadmium pharmacology
Oxidative Stress
Phaseolus metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0981-9428
- Volume :
- 43
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Plant physiology and biochemistry : PPB
- Publication Type :
- Academic Journal
- Accession number :
- 15890519
- Full Text :
- https://doi.org/10.1016/j.plaphy.2005.03.007