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Discrete serum protein signatures discriminate between human retrovirus-associated hematologic and neurologic disease.
- Source :
-
Leukemia [Leukemia] 2005 Jul; Vol. 19 (7), pp. 1229-38. - Publication Year :
- 2005
-
Abstract
- The human T-cell leukemia virus type I (HTLV-I) is the causative agent for adult T-cell leukemia (ATL) and HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Approximately 5% of infected individuals will develop either disease and currently there are no diagnostic tools for early detection or accurate assessment of disease state. We have employed high-throughput expression profiling of serum proteins using mass spectrometry to identify protein expression patterns that can discern between disease states of HTLV-I-infected individuals. Our study group consisted of 42 ATL, 50 HAM/TSP, and 38 normal controls. Spectral peaks corresponding to peptide ions were generated from MS-TOF data. We applied Classification and Regression Tree analysis to build a decision algorithm, which achieved 77% correct classification rate across the three groups. A second cohort of 10 ATL, 10 HAM and 10 control samples was used to validate this result. Linear discriminate analysis was performed to verify and visualize class separation. Affinity and sizing chromatography coupled with tandem mass spectrometry was used to identify three peaks specifically overexpressed in ATL: an 11.7 kDa fragment of alpha trypsin inhibitor, and two contiguous fragments (19.9 and 11.9 kDa) of haproglobin-2. To the best of our knowledge, this is the first application of protein profiling to distinguish between two disease states resulting from a single infectious agent.
- Subjects :
- Adolescent
Adult
Aged
Aged, 80 and over
Cohort Studies
Diagnosis, Differential
Female
Humans
Leukemia-Lymphoma, Adult T-Cell diagnosis
Male
Middle Aged
Paraparesis, Tropical Spastic diagnosis
Predictive Value of Tests
Regression Analysis
Reproducibility of Results
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods
Blood Proteins analysis
Leukemia-Lymphoma, Adult T-Cell blood
Paraparesis, Tropical Spastic blood
Subjects
Details
- Language :
- English
- ISSN :
- 0887-6924
- Volume :
- 19
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Leukemia
- Publication Type :
- Academic Journal
- Accession number :
- 15889159
- Full Text :
- https://doi.org/10.1038/sj.leu.2403781