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The effects of 3-methylcholanthrene on lymphocyte proliferation in the common carp (Cyprinus carpio L.).
- Source :
-
Toxicology [Toxicology] 2005 Jul 01; Vol. 211 (1-2), pp. 156-64. Date of Electronic Publication: 2005 Mar 25. - Publication Year :
- 2005
-
Abstract
- The sensitivity of lymphocyte proliferation as bioindicator of pollution stress was evaluated in the common carp (Cyrinus carpio L.). The time course response of peripheral blood leukocyte proliferation in response or not to mitogens was measured from 1 to 7 days after peritoneal injection of 3-methylcholantrene (3-MC), and compared to the time course response of a highly sensitive biomarker, induction of cytochrome P450. 3-Methylcholanthrene (40 mg kg(-1)) inhibited both B- and T-lymphocyte proliferation in response to lipopolysaccharide (LPS) and concanavalin A (Con A). Studies with alpha-naphtofiavone, suggest the lack of metabolic processes. 3-Methylcholanthrene alone strongly stimulated resting peripheral blood leukocytes (PBLs) proliferation. This effect was not transient. The induction of lymphocyte proliferation paralleled the increase in cytochrome P450 content in the liver. The specificity of polycyclic aromatic hydrocarbon (PAH)-induced lymphocyte proliferation suggests that this immune activity may be an early marker of exposure to PAHs in aquatic environments. The capacity of 3-MC to induce rapid lymphocyte proliferation may be related to PAH-induced rapid clonal expansion in mammals. These results strongly suggested that the underlying mechanism might be the same in both models. More studies are needed in fish to explain this phenomenon and may be helpful in understanding the occurrence of neoplastic epizootics in fish associated with PAH exposition.
- Subjects :
- Animals
Benzoflavones pharmacology
Cell Proliferation drug effects
Cell Separation
Cytochrome P-450 Enzyme Inhibitors
Cytochrome P-450 Enzyme System metabolism
Enzyme Inhibitors pharmacology
Injections, Intraperitoneal
Liver drug effects
Liver enzymology
Methylcholanthrene administration & dosage
Methylcholanthrene pharmacokinetics
Microsomes drug effects
Microsomes metabolism
Mitogens pharmacology
Receptors, Aryl Hydrocarbon antagonists & inhibitors
Subcellular Fractions drug effects
Subcellular Fractions metabolism
Carps immunology
Immunity, Cellular drug effects
Lymphocytes drug effects
Lymphocytes immunology
Methylcholanthrene toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 0300-483X
- Volume :
- 211
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 15863259
- Full Text :
- https://doi.org/10.1016/j.tox.2005.02.015