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[Screening and identification of a strain for hydroxylation of nicotinic acid].
- Source :
-
Wei sheng wu xue bao = Acta microbiologica Sinica [Wei Sheng Wu Xue Bao] 2005 Feb; Vol. 45 (1), pp. 6-9. - Publication Year :
- 2005
-
Abstract
- Strain screening was conducted on a large scale in order to develop a biotransforming process for 6-hydroxynicotinic acid production. A strain named as NA-1 was isolated from soil which could transform nicotinic acid into a new product showed by HPLC. The new product was identified as 6-hydroxynicotinic acid by MS and 1H-NMR. The morphology, physiological and biochemical characteristics of strain NA-1 were studied and showed that characteristics of strain NA-1 were essentially consistent with Pseudomonas putida. The analysis of 16S rDNA sequence from strain NA-1 suggested that strain NA-1 was clustered together with P. putida in phylogenetic tree and the sequence identity between stain NA-1 and Pseudomonas putida was 99%. So strain NA-1 was identified to P. putida.
- Subjects :
- Chromatography, High Pressure Liquid
DNA, Bacterial genetics
Hydroxylation
Phylogeny
Pseudomonas putida genetics
Pseudomonas putida isolation & purification
RNA, Ribosomal, 16S genetics
Linoleic Acid metabolism
Pseudomonas putida classification
Pseudomonas putida metabolism
Soil Microbiology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0001-6209
- Volume :
- 45
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Wei sheng wu xue bao = Acta microbiologica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 15847152