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Evaluation of Salmonella typhimurium strains harbouring defined mutations in htrA and aroA in the murine salmonellosis model.
- Source :
-
Microbial pathogenesis [Microb Pathog] 1992 Feb; Vol. 12 (2), pp. 145-51. - Publication Year :
- 1992
-
Abstract
- Derivatives of the mouse-virulent Salmonella typhimurium strain SL1344 were constructed harbouring defined mutations in htrA, aroA or htrA aroA combined. When administered orally or intravenously to BALB/c mice, all the mutants were found to be highly attenuated. All mutants were able to confer significant protection against lethal challenge with SL1344 after a single oral dose of live organisms. SL1344 htrA mutants persisted in livers and spleens at a lower level than SL1344 aroA mutants after intravenous administration. SL1344 htrA aroA mutants persisted at an even lower level and were cleared from the livers and spleens of mice within 21 days of intravenous administration. Thus htrA and htrA aroA mutants can be considered as potential oral vaccines against salmonellosis.
- Subjects :
- Animals
Bacterial Vaccines genetics
Bacterial Vaccines immunology
Blotting, Southern
Cloning, Molecular
Disease Models, Animal
Heat-Shock Proteins immunology
Kinetics
Mice
Mice, Inbred BALB C
Restriction Mapping
Salmonella Infections, Animal prevention & control
Salmonella typhimurium pathogenicity
Vaccines, Attenuated genetics
Vaccines, Attenuated immunology
Virulence genetics
Heat-Shock Proteins genetics
Mutation
Salmonella Infections, Animal microbiology
Salmonella typhimurium genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0882-4010
- Volume :
- 12
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Microbial pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 1584006
- Full Text :
- https://doi.org/10.1016/0882-4010(92)90117-7