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Zfp36l3, a rodent X chromosome gene encoding a placenta-specific member of the Tristetraprolin family of CCCH tandem zinc finger proteins.
- Source :
-
Biology of reproduction [Biol Reprod] 2005 Aug; Vol. 73 (2), pp. 297-307. Date of Electronic Publication: 2005 Apr 06. - Publication Year :
- 2005
-
Abstract
- Members of the tristetraprolin (TTP) family of CCCH tandem zinc finger (TZF) proteins can bind directly to AU-rich elements (ARE) in mRNA, causing deadenylation and destabilization of the transcripts to which they bind. We describe here a novel fourth mammalian member of the TTP protein family, designated ZFP36L3, which could also bind directly to ARE-containing RNAs and could promote the deadenylation and degradation of ARE-containing target RNAs. Zfp36l3 transcript expression was detected only in placenta and extraembryonic tissues in the mouse. It was expressed throughout development in the placenta and was particularly highly expressed in the cells of the labyrinthine layer of the trophoblastic placenta. Unlike the other family members, the expression of a ZFP36L3-green fluorescent protein fusion protein was entirely cytoplasmic when expressed in 293 cells, even in the presence of the CRM1-dependent nuclear export inhibitor leptomycin B. Zfp36l3 was located on the mouse X chromosome; a similar predicted gene was present on the rat X chromosome, but there was no evidence for a similar gene in humans. ZFP36L3 may thus be a rodent-specific or even murine-specific member of the TTP protein family. Its presumed role in placental physiology may be unique to rodents or murine rodents, but this role may be subsumed by other family members in nonrodents.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Cell Nucleus metabolism
Cytoplasm metabolism
DNA-Binding Proteins biosynthesis
DNA-Binding Proteins metabolism
Electrophoretic Mobility Shift Assay
Female
Immediate-Early Proteins biosynthesis
Immediate-Early Proteins metabolism
In Situ Hybridization
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Placenta metabolism
Pregnancy
RNA, Messenger biosynthesis
RNA, Messenger genetics
RNA, Messenger metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment
Tandem Repeat Sequences
Transfection
Tristetraprolin
Tumor Necrosis Factor-alpha metabolism
X Chromosome genetics
DNA-Binding Proteins genetics
Immediate-Early Proteins genetics
Placenta physiology
Zinc Fingers genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3363
- Volume :
- 73
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biology of reproduction
- Publication Type :
- Academic Journal
- Accession number :
- 15814898
- Full Text :
- https://doi.org/10.1095/biolreprod.105.040527