Back to Search
Start Over
MUC5B glycosylation in human saliva reflects blood group and secretor status.
- Source :
-
Glycobiology [Glycobiology] 2005 Aug; Vol. 15 (8), pp. 791-804. Date of Electronic Publication: 2005 Apr 06. - Publication Year :
- 2005
-
Abstract
- This study aimed to characterize human salivary glycoforms and the natural glycosylation variation of the major ABO blood group bearing high molecular weight glycoprotein fraction MG1, which mainly consists of MUC5B mucin. Reduced and alkylated mucins from individuals of blood group A, B, and O were purified by sodium dodecyl sulfate-agarose/polyacrylamide composite gel electrophoresis (SDS-AgPAGE), blotted to polyvinylidene fluoride (PVDF) membranes, and visualized with alcian blue. O-linked oligosaccharides were released from MUC5B glycoform bands by reductive beta-elimination and analyzed by liquid chromatography (LC) electrospray ion trap mass spectrometry (MS). Slow electrophoretically migrating MUC5B components (sm) were found to be dominated by neutral oligosaccharides, and fast-migrating (fm) components were dominated by sulfated oligosaccharides. ABO blood group-specific sequences were found on all glycoforms, and novel oligosaccharides containing blood group A and B type sequences were sequenced. This is the first molecular description of the influence of the blood group ABO system on salivary MUC5B oligosaccharides. Expanding these results from the three A, B, and O individuals into larger population (29 individuals), we found oligosaccharide sequences corresponding to the blood group of the donor on MUC5B from 23 individuals. The remaining six individuals were characterized by a high degree of sialylation. These individuals were assigned as nonsecretors, whereas blood group-expressing individuals were assigned as secretors. Western blot assays with antibodies confirmed increased expression of Sialyl Lewis a (Si-Le(a)) in the nonsecretors. Our results highlight that salivary MUC5B consists of glycoforms with distinct glycosylation that vary extensively between individuals and that some of this variation is owing to blood group and secretor status.
- Subjects :
- Amino Sugars analysis
Amino Sugars chemistry
Antigens, Neoplasm immunology
Antigens, Neoplasm metabolism
Carbohydrate Conformation
Carbohydrate Sequence
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Glycosylation
Humans
Molecular Sequence Data
Monosaccharides analysis
Monosaccharides chemistry
Mucin-5B
Mucins chemistry
Mucins isolation & purification
Oligosaccharides analysis
Oligosaccharides immunology
Oligosaccharides metabolism
Sialyl Lewis X Antigen
Spectrometry, Mass, Fast Atom Bombardment
ABO Blood-Group System
Mucins metabolism
Oligosaccharides chemistry
Saliva metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0959-6658
- Volume :
- 15
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Glycobiology
- Publication Type :
- Academic Journal
- Accession number :
- 15814823
- Full Text :
- https://doi.org/10.1093/glycob/cwi059