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Molecular detection of early-stage laryngopharyngeal squamous cell carcinomas.
- Source :
-
Clinical cancer research : an official journal of the American Association for Cancer Research [Clin Cancer Res] 2005 Apr 01; Vol. 11 (7), pp. 2547-51. - Publication Year :
- 2005
-
Abstract
- Purpose: We sought to determine whether early-stage laryngopharyngeal squamous cell carcinomas (SCC) can be detected through molecular analysis of exfoliated cells collected with the use of a pharyngoesophageal brush (PEB).<br />Experimental Design: Thirty-three patients with a single, untreated, early-stage (T1 or T2) SCC of the supraglottic larynx or pharynx underwent collection of cells with a PEB, followed by endoscopic biopsy of the tumor. PEB specimens were also collected from five healthy subjects. PEB samples and tumor tissue were examined for hypermethylation of p16INK4a (CDKN2) gene promoter CpG islands (assayed by methylation-specific PCR) and UT5085 tetranucleotide microsatellite instability (assayed by GeneScan analysis). PEB samples were also subjected to cytologic analysis.<br />Results: Eight of 33 (24%) tumors exhibited a bandshift at UT5085, and 14 of 33 (42%) exhibited hypermethylation at the p16 promoter. Overall, 17 of 33 (52%) patients had at least one of the two markers in their tumor. Cytologic analysis of PEB samples revealed tumor in 4 of 33 (12%) patients; cytologic findings were normal in all five control subjects. Molecular analysis of PEB samples revealed tumor DNA in 13 of 17 (76%) patients with at least one of the two molecular markers in their tumor. Eight of 14 (57%) patients with p16 hypermethylation in their tumor and 8 of 8 (100%) patients with UT5085 microsatellite instability in their tumor had similar findings in the PEB samples. None of the PEB samples from the control subjects or patients with neither molecular marker in their tumor displayed abnormality.<br />Conclusion: Molecular analysis of PEB samples holds promise for the early detection of early-stage laryngopharyngeal SCCs. New molecular markers need to be identified to increase the sensitivity of molecular screening.
- Subjects :
- Carcinoma, Squamous Cell genetics
Cell Line, Tumor
CpG Islands genetics
Cyclin-Dependent Kinase Inhibitor p16 genetics
DNA Methylation
Humans
Hypopharyngeal Neoplasms genetics
Laryngeal Neoplasms genetics
Microsatellite Repeats genetics
Neoplasm Staging methods
Pharyngeal Neoplasms genetics
Promoter Regions, Genetic genetics
Prospective Studies
Carcinoma, Squamous Cell pathology
Hypopharyngeal Neoplasms pathology
Laryngeal Neoplasms pathology
Pharyngeal Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1078-0432
- Volume :
- 11
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Clinical cancer research : an official journal of the American Association for Cancer Research
- Publication Type :
- Academic Journal
- Accession number :
- 15814632
- Full Text :
- https://doi.org/10.1158/1078-0432.CCR-04-1535