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Immune responses to recombinants of the South African vaccine strain of lumpy skin disease virus generated by using thymidine kinase gene insertion.
- Source :
-
Vaccine [Vaccine] 2005 Apr 27; Vol. 23 (23), pp. 3061-7. - Publication Year :
- 2005
-
Abstract
- The South African vaccine strain of lumpy skin disease virus (type SA-Neethling) is currently being developed as a vector for recombinant vaccines of economically important livestock diseases throughout Africa. In this study, the feasibility of using the viral thymidine kinase gene as the site of insertion was investigated and recombinant viruses were evaluated in animal trials. Two separate recombinants were generated and selected for homogeneity expressing either the structural glycoprotein gene of bovine ephemeral fever virus (BEFV) or the two structural glycoprotein genes of Rift Valley fever virus (RVFV). Both recombinants incorporate the enhanced green fluorescent protein (EGFP) as a visual marker and the Escherichia coli guanine phosphoribosyl transferase (gpt) gene for dominant positive selection. The LSDV-RVFV recombinant construct (rLSDV-RVFV) protected mice against virulent RVFV challenge. In a small-scale BEFV-challenge cattle trial the rLSDV-BEFV construct failed to fully protect the cattle against virulent challenge, although both a humoral and cellular BEFV-specific immune response was elicited.
- Subjects :
- Animals
Antibodies, Viral blood
Body Temperature
Genetic Vectors
Lumpy skin disease virus immunology
Lymphocyte Activation
Mice
Mice, Inbred BALB C
Ephemeral Fever Virus, Bovine immunology
Lumpy skin disease virus genetics
Rift Valley fever virus immunology
Thymidine Kinase genetics
Vaccines, Synthetic immunology
Viral Vaccines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0264-410X
- Volume :
- 23
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 15811653
- Full Text :
- https://doi.org/10.1016/j.vaccine.2004.10.006