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The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1.
- Source :
-
Cell [Cell] 2005 Mar 25; Vol. 120 (6), pp. 773-88. - Publication Year :
- 2005
-
Abstract
- Cohesion established between sister chromatids during pre-meiotic DNA replication mediates two rounds of chromosome segregation. The first division is preceded by an extended prophase wherein homologous chromosomes undergo recombination. The persistence of cohesion during prophase is essential for recombination and both meiotic divisions. Here we show that Mnd2, a subunit of the anaphase-promoting complex (APC/C) from budding yeast, is essential to prevent premature destruction of cohesion in meiosis. During S- and prophase, Mnd2 prevents activation of the APC/C by a meiosis-specific activator called Ama1. In cells lacking Mnd2 the APC/C-Ama1 enzyme triggers degradation of Pds1, which causes premature sister chromatid separation due to unrestrained separase activity. In vitro, Mnd2 inhibits ubiquitination of Pds1 by APC/C-Ama1 but not by other APC/C holo-enzymes. We conclude that chromosome segregation in meiosis depends on the selective inhibition of a meiosis-specific form of the APC/C.
- Subjects :
- Anaphase genetics
Anaphase physiology
Anaphase-Promoting Complex-Cyclosome
CDC2 Protein Kinase genetics
CDC2 Protein Kinase metabolism
CDC28 Protein Kinase, S cerevisiae genetics
CDC28 Protein Kinase, S cerevisiae metabolism
Cdc20 Proteins
Cell Cycle Proteins genetics
Chromatids genetics
Chromatids metabolism
Chromosome Segregation genetics
Endopeptidases metabolism
Meiosis genetics
Nuclear Proteins genetics
Nuclear Proteins metabolism
Protein Denaturation physiology
Saccharomyces cerevisiae cytology
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins genetics
Securin
Separase
Ubiquitin-Protein Ligase Complexes genetics
Cell Cycle Proteins metabolism
Chromosome Segregation physiology
Meiosis physiology
Saccharomyces cerevisiae Proteins metabolism
Ubiquitin-Protein Ligase Complexes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0092-8674
- Volume :
- 120
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Cell
- Publication Type :
- Academic Journal
- Accession number :
- 15797379
- Full Text :
- https://doi.org/10.1016/j.cell.2005.01.032