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Detection of influenza A virus by RT-PCR and standard methods in experimental infection of Ducks.
- Source :
-
The new microbiologica [New Microbiol] 2005 Jan; Vol. 28 (1), pp. 31-5. - Publication Year :
- 2005
-
Abstract
- Cloacal swabs collected from mallard ducks (Anas platyrhynchos) experimentally infected with a H7N1 avian influenza strain were examined by Reverse Transcription Polymerase Chain Reaction to detect the influenza A virus. Reverse Transcription Polymerase Chain Reaction was compared with standard methods: inoculation of embryonated chicken eggs and inoculation of three established cell lines: Newborn Swine Kidney cells, Newborn Pig Trachea cells and Madine Darby Canine Kidney cells. Reverse Transcription Polymerase Chain Reaction was performed using a set of primers based on conserved regions of the matrix and nucleoprotein genes.
Details
- Language :
- English
- ISSN :
- 1121-7138
- Volume :
- 28
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The new microbiologica
- Publication Type :
- Academic Journal
- Accession number :
- 15782624