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Decrease in pathology and progression of scrapie after immunisation with synthetic prion protein peptides in hamsters.
- Source :
-
Vaccine [Vaccine] 2005 Apr 22; Vol. 23 (22), pp. 2862-8. - Publication Year :
- 2005
-
Abstract
- Effective therapy for prion diseases is currently unavailable. Recently, vaccination was shown to be effective in mouse models of a particular neurodegenerative conditions: Alzheimer's disease (AD). Here, we report that vaccination with synthetic oligopeptides homologous to the hamster (Mesocricetus auratus) prion protein augments survival time in animals infected intraperitoneally with 263K scrapie agent. For each hamster included in the study, prion-specific serum antibodies as well as deposition of pathological prion protein (PrP(res)), glial fibrillary acidic protein (GFAP), and mRNA expression for cytokines (TNF alpha, IL-1beta, IL-10) in brain tissues were evaluated. In immunized animals, increased survival after challenge was associated with a reduction of cerebral lesion, PrP deposition and GFAP expression; in these animals, anti-prion protein peptide antibody levels were increased, and the expression of pro-inflammatory cytokines (TNF alpha and IL-1beta) was reduced. Vaccination could be an effective therapeutic approach to postpone disease onset.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Cricetinae
DNA Primers
Enzyme-Linked Immunosorbent Assay
Female
Immunohistochemistry
Interleukin-1 genetics
Interleukin-1 metabolism
Interleukin-10 metabolism
Mesocricetus
Molecular Sequence Data
Peptides chemistry
Prions chemistry
RNA, Messenger metabolism
Scrapie prevention & control
Tumor Necrosis Factor-alpha genetics
Peptides administration & dosage
Prions administration & dosage
Scrapie pathology
Subjects
Details
- Language :
- English
- ISSN :
- 0264-410X
- Volume :
- 23
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 15780734
- Full Text :
- https://doi.org/10.1016/j.vaccine.2004.11.067