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The effect of simulated microgravity on hybridoma cells.
- Source :
-
Acta astronautica [Acta Astronaut] 2005 Apr; Vol. 56 (8), pp. 721-8. - Publication Year :
- 2005
-
Abstract
- The effect of clinostat-simulated microgravity on SP-2/0 and 1D6 hybridoma cells was studied. Clinorotation during 4-5 days at 1.5 rounds per minute decreased dramatically their proliferating capacity: the rotated cells divided less than once while control cells performed 4-5 divisions. They decreased the non-specific adhesion to tissue culture plastic, but increased the number of cell-to-cell contacts. Such phenomenological changes were accompanied with the alterations in pericellular glycosaminoglycans: decreased accumulation of hyaluronic acid and increased accumulation of chondroitin/dermatan-sulfate, as well as with the increase of cytoplasmic Ca2+ concentration. Clinorotation resulted in hybridoma nicotinic receptor desensitization but not down-regulation. In contrast, both the quantity and quality (molecular isoforms, affinity and specificity) of the antibody produced by 1D6 hybridoma cells were not altered by clinorotation. It is concluded that simulated microgravity affected the proliferating and adhesive, but not biosynthetic properties of hybridoma cells.<br /> (c2005 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Antibody Formation
Calcium metabolism
Cell Adhesion physiology
Cell Line
Chondroitin biosynthesis
Cytoplasm physiology
Dermatan Sulfate biosynthesis
Down-Regulation physiology
Gravitation
Hyaluronic Acid biosynthesis
Hybridomas metabolism
Mice
Nicotine pharmacology
Rats
Receptors, Nicotinic drug effects
Rotation
Cell Proliferation
Hybridomas cytology
Weightlessness Simulation
Subjects
Details
- Language :
- English
- ISSN :
- 0094-5765
- Volume :
- 56
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Acta astronautica
- Publication Type :
- Academic Journal
- Accession number :
- 15779143
- Full Text :
- https://doi.org/10.1016/j.actaastro.2004.12.003