Back to Search
Start Over
Anti-tuberculosis constituents from the stem bark of Micromelum hirsutum.
- Source :
-
Planta medica [Planta Med] 2005 Mar; Vol. 71 (3), pp. 261-7. - Publication Year :
- 2005
-
Abstract
- Anti-TB bioassay-directed fractionation led to the isolation of six carbazole alkaloids, as well as the gamma-lactone derivative of oleic acid, from the CH (2)Cl (2) extract of the stem bark of Micromelum hirsutum. The carbazoles include the new micromeline ( 2) and five known alkaloids: lansine ( 3), 3-methylcarbazole ( 4), methyl carbazole-3-carboxylate ( 5), 3-formylcarbazole ( 6), and 3-formyl-6-methoxycarbazole ( 7). Compound 1 was identified as the lactone derivative of oleic acid, (-)- Z-9-octadecene-4-olide, for which the trivial name micromolide ( 1) is suggested. It showed potent in vitro anti-TB activity against H37R v (MIC: 1.5 microg/mL), a selectivity index (SI) of 63, and exhibited activity against the Erdman strain of M. tuberculosis in a J774 mouse macrophage model (EC (90) : 5.6 microg/mL). Thus, 1 appears worthy of further evaluation as a potential new anti-TB agent. Isolates 2, 3, 6 and 7 had anti-TB MIC values between 14.3 and 42.3 microg/mL, while compounds 4 and 5 were considered inactive (MIC > 128 microg/mL). Structure elucidation and identification were based on spectroscopic analysis, including MS, 1D/2D NMR, and a full (1)H spin system analysis of 1.
- Subjects :
- Animals
Antitubercular Agents administration & dosage
Antitubercular Agents therapeutic use
Humans
Macrophages microbiology
Mice
Microbial Sensitivity Tests
Mycobacterium tuberculosis pathogenicity
Plant Bark
Plant Extracts administration & dosage
Plant Extracts therapeutic use
Antitubercular Agents pharmacology
Mycobacterium tuberculosis drug effects
Phytotherapy
Plant Extracts pharmacology
Rutaceae
Subjects
Details
- Language :
- English
- ISSN :
- 0032-0943
- Volume :
- 71
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Planta medica
- Publication Type :
- Academic Journal
- Accession number :
- 15770548
- Full Text :
- https://doi.org/10.1055/s-2005-837826