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Isolation of Waddlia malaysiensis, a novel intracellular bacterium, from fruit bat (Eonycteris spelaea).
- Source :
-
Emerging infectious diseases [Emerg Infect Dis] 2005 Feb; Vol. 11 (2), pp. 271-7. - Publication Year :
- 2005
-
Abstract
- An obligate intracellular bacterium was isolated from urine samples from 7 (3.5%) of 202 fruit bats (Eonycteris spelaea) in peninsular Malaysia. The bacterium produced large membrane-bound inclusions in human, simian, and rodent cell lines, including epithelial, fibroblastlike, and lymphoid cells. Thin-section electron microscopy showed reticulate bodies dividing by binary fission and elementary bodies in the inclusions; mitochondria surrounded the inclusions. The inclusions were positive for periodic acid-Schiff stain but could not be stained by fluorescein-labeled anti-Chlamydia trachomatis major outer membrane protein monoclonal antibody. The bacterium was resistant to penicillin and streptomycin (MICs > 256 mg/L) but susceptible to tetracycline (MIC = 0.25 mg/L) and chloramphenicol (MIC = 0.5 mg/L). Sequence analysis of the 16SrRNA gene indicated that it was most closely related to 2 isolates of Waddlia chondrophila (94% and 96% identity). The 16S and 23S rRNA gene signatures were only 91% identical. We propose this novel bacterium be called W. malaysiensis.
- Subjects :
- 3T3 Cells
Acetylmuramyl-Alanyl-Isoglutamine chemistry
Acetylmuramyl-Alanyl-Isoglutamine genetics
Animals
Anti-Bacterial Agents pharmacology
Base Sequence
Chiroptera urine
Chlamydiales drug effects
Chlamydiales genetics
Chlamydiales ultrastructure
Chlorocebus aethiops
Cricetinae
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal Spacer chemistry
DNA, Ribosomal Spacer genetics
Humans
Mice
Microbial Sensitivity Tests
Microscopy, Electron
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S chemistry
RNA, Ribosomal, 16S genetics
RNA, Ribosomal, 23S chemistry
RNA, Ribosomal, 23S genetics
Sequence Alignment
Vero Cells
Acetylmuramyl-Alanyl-Isoglutamine analogs & derivatives
Chiroptera microbiology
Chlamydiales isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1080-6040
- Volume :
- 11
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Emerging infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 15752446
- Full Text :
- https://doi.org/10.3201/eid1102.040746