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Reovirus serotype 1/strain Lang-stimulated activation of antigen-specific T lymphocytes in Peyer's patches and distal gut-mucosal sites: activation status and cytotoxic mechanisms.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2005 Mar 15; Vol. 174 (6), pp. 3580-9. - Publication Year :
- 2005
-
Abstract
- Intraduodenal priming of mice with reovirus serotype 1/strain Lang (reovirus 1/L) stimulates gut lymphocytes and generates precursor and effector CTLs. Our earlier studies demonstrated that germinal center and T cell Ag (GCT) is a marker which identifies reovirus 1/L-specific precursor CTL and effector CTL in Peyer's patches (PP) of reovirus 1/L-inoculated mice. In this study, we characterized the expression of the activation markers, GCT and CD11c, on reovirus 1/L-stimulated gut lymphocytes and the effector mechanisms involved in reovirus 1/L-specific cytotoxicity. We found that intraduodenal reovirus 1/L inoculation of mice induced the expression of both GCT and CD11c on PP lymphocytes (PPL), intraepithelial lymphocytes (IEL), and lamina propria lymphocytes (LPL), and these activated cells expressed Fas ligand (FasL). The majority of the GCT+ CD11c+ IEL and LPL expressed a phenotype, TCRalphabeta+ Thy-1+ CD8+ similar to that expressed on reovirus 1/L-stimulated PPL. However, splenic lymphocytes expressed GCT but not CD11c after stimulation with reovirus 1/L. Perforin, Fas-FasL, and TRAIL pathways were found to be involved in PPL, IEL, and LPL cytotoxic activity against reovirus 1/L-infected targets. In PPL, perforin and Fas-FasL pathways were more effective than TRAIL. In IEL, all three cytotoxic mechanisms were equally as effective. However, LPL prefer Fas-FasL and TRAIL over perforin. Further, we demonstrated the preferential migration of GCT+ PPL to the intraepithelial compartment and the lamina propria. These results suggest that GCT and CD11c can be used as activation markers for gut lymphocytes and CD11c can also be used to differentiate between activated gut and systemic lymphocytes.
- Subjects :
- Animals
Apoptosis Regulatory Proteins
Base Sequence
CD11c Antigen biosynthesis
Cytotoxicity, Immunologic
DNA genetics
Duodenum cytology
Duodenum immunology
Duodenum virology
Fas Ligand Protein
Female
Gene Expression
Immunity, Mucosal
Lymphocyte Activation
Membrane Glycoproteins genetics
Membrane Proteins genetics
Mice
Mice, Inbred BALB C
Orthoreovirus, Mammalian pathogenicity
Peyer's Patches immunology
Peyer's Patches virology
Pore Forming Cytotoxic Proteins
Reoviridae Infections genetics
Reoviridae Infections immunology
TNF-Related Apoptosis-Inducing Ligand
Tumor Necrosis Factor-alpha genetics
Orthoreovirus, Mammalian immunology
T-Lymphocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 174
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 15749895
- Full Text :
- https://doi.org/10.4049/jimmunol.174.6.3580