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Matrix metalloproteinase-12 expression correlates with local recurrence and metastatic disease in non-small cell lung cancer patients.
- Source :
-
Clinical cancer research : an official journal of the American Association for Cancer Research [Clin Cancer Res] 2005 Feb 01; Vol. 11 (3), pp. 1086-92. - Publication Year :
- 2005
-
Abstract
- Purpose: Non-small cell lung cancer (NSCLC) is a very common and aggressive malignancy. Survival after resection of tumor is especially determined by the occurrence of distant metastasis. Matrix metalloproteinases (MMP) support this metastatic process by degradation of the extracellular matrix.<br />Experimental Design: We used DNA microarray technology to examine the expression of 22 MMPs in 89 surgically treated NSCLC patients. Validation of microarray results was done using reverse transcription-PCR and immunohistology.<br />Results: MMP-1, MMP-9, and MMP-12 expression was significantly increased in tumors versus corresponding lung tissues. MMP-12 expression significantly correlated with local recurrence and metastatic disease. Multivariate Cox regression analysis revealed MMP-12 expression as an independent prognostic factor for tumor relapse-free interval. Gene expression analysis of 158 healthy tissues from 32 different organs revealed no MMP-12 expression in these organs and immunohistology identified MMP-12 protein in NSCLC only in tumor cells.<br />Conclusions: MMP-12 might be not only a prognostic marker, but also a valuable therapeutic target.
- Subjects :
- Aged
Carcinoma, Non-Small-Cell Lung genetics
Carcinoma, Non-Small-Cell Lung metabolism
Female
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Lung Neoplasms genetics
Lung Neoplasms metabolism
Male
Matrix Metalloproteinase 12
Metalloendopeptidases analysis
Neoplasm Metastasis
Neoplasm Recurrence, Local
Neoplasm Staging
Oligonucleotide Array Sequence Analysis
RNA, Neoplasm genetics
RNA, Neoplasm metabolism
Reverse Transcriptase Polymerase Chain Reaction
Survival Analysis
Carcinoma, Non-Small-Cell Lung pathology
Lung Neoplasms pathology
Metalloendopeptidases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1078-0432
- Volume :
- 11
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Clinical cancer research : an official journal of the American Association for Cancer Research
- Publication Type :
- Academic Journal
- Accession number :
- 15709175