Back to Search
Start Over
T cells from epicutaneously immunized mice are prone to T cell receptor revision.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2005 Feb 22; Vol. 102 (8), pp. 2898-903. Date of Electronic Publication: 2005 Feb 11. - Publication Year :
- 2005
-
Abstract
- Epicutaneous immunization of T cell receptor (TCR) transgenic (Tg) mice whose CD4(+) T cells are specific for the Ac1-11 fragment of myelin basic protein (MBP) with Ac1-11 elicits T cells with dominant suppressor/regulatory activity that confers protection against Ac1-11-induced experimental autoimmune encephalomyelitis. We now report that such disease-resistant MBP TCR Tg mice also harbor a sizeable fraction of peripheral CD4(+) T cells lacking surface expression of the Tg TCR beta chain and expressing diverse, endogenously rearranged TCR beta chains. Ex vivo incubation at physiological temperature caused the loss of neo-beta-chain expression and reversion to the MBP alphabeta TCR(+) phenotype. The presence of recombination activating gene 1 and 2 proteins in CD4(+) T cells with revised TCRs was consistent with effective V(D)J recombination activity. The emergence of these cells did not depend on the thymic compartment. We conclude that in mice epicutaneously immunized with an autoantigen, peripheral specific T cells are susceptible to multiple mechanisms of tolerance.
- Subjects :
- Animals
Cells, Cultured
DNA-Binding Proteins analysis
Encephalomyelitis, Autoimmune, Experimental prevention & control
Homeodomain Proteins analysis
Immunization
Immunophenotyping
Mice
Mice, Transgenic
Peptide Fragments immunology
Receptors, Antigen, T-Cell, alpha-beta analysis
Autoantigens immunology
CD4-Positive T-Lymphocytes immunology
Immune Tolerance
Myelin Basic Protein immunology
Receptors, Antigen, T-Cell, alpha-beta physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 102
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 15708975
- Full Text :
- https://doi.org/10.1073/pnas.0409880102