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Oxidative DNA damage in peripheral leukocytes of mild cognitive impairment and AD patients.
- Source :
-
Neurobiology of aging [Neurobiol Aging] 2005 May; Vol. 26 (5), pp. 567-73. - Publication Year :
- 2005
-
Abstract
- It is well established that oxidative stress plays a key role in the degenerative neuronal death and progression of Alzheimer's disease (AD), although it is not clear if it is the primary triggering event in the pathogenesis of this disorder. Mild cognitive impairment (MCI) is a clinical condition between normal aging and AD, characterized by a memory deficit without loss of general cognitive and functional abilities. We performed this study by a comet assay analysis to evaluate the level of primary and oxidative DNA damage in two groups of MCI and AD patients, compared to healthy controls. Data showed a significantly higher level of primary DNA damage in leukocytes of AD and also of MCI patients compared to control individuals (average: 2.09+/-0.79 and 2.47+/-1.01, respectively for AD and MCI, versus 1.04+/-0.31 in controls). Moreover, the amount of oxidised DNA bases (both purines and pyrimidines) was significatively higher in the two groups of patients (AD and MCI) compared to controls. Our results give a further indication that oxidative stress, at least at the DNA level, is an earlier event in the pathogenesis of AD.
- Subjects :
- Aged
Alzheimer Disease physiopathology
Comet Assay methods
DNA Damage drug effects
DNA-Formamidopyrimidine Glycosylase
Deoxyadenosines
Deoxyribonuclease (Pyrimidine Dimer)
Electrophoresis
Escherichia coli Proteins
Female
Humans
Leukocytes, Mononuclear drug effects
Male
Memory Disorders etiology
Middle Aged
Purines metabolism
Pyrimidines metabolism
Alzheimer Disease pathology
DNA Damage physiology
Leukocytes, Mononuclear metabolism
Memory Disorders pathology
Oxidative Stress
Subjects
Details
- Language :
- English
- ISSN :
- 0197-4580
- Volume :
- 26
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Neurobiology of aging
- Publication Type :
- Academic Journal
- Accession number :
- 15708428
- Full Text :
- https://doi.org/10.1016/j.neurobiolaging.2004.07.016