Back to Search
Start Over
A study on the bacterial photo-toxicity of phenothiazinium based photosensitisers.
- Source :
-
FEMS immunology and medical microbiology [FEMS Immunol Med Microbiol] 2005 Mar 01; Vol. 43 (3), pp. 367-72. - Publication Year :
- 2005
-
Abstract
- "Comet assay" showed light activated (3.15 Jcm-2 over 30 min) phenothiazinium based photosensitisers (PhBPs) to induce photo-damage of Staphylococcus aureus DNA, as indicated by DNA "tails" between 80 and 120 microm. In general, PhBPs exhibited significant singlet oxygen yields (Phi(DeltaPhBP)>0.7), suggesting the use of type II mechanisms of photo-oxidation. However, the photodynamic action of PhBPs on DNA showed generally insignificant production of 7,8-dihydro-8-oxo-2'-deoxyguanosine, normally a major product of type II DNA photo-oxidation. These combined results show DNA to be a major site of action of PhBPs and suggest that this action may involve type II attack on a nucleoside(s) other than guanosine.
- Subjects :
- 8-Hydroxy-2'-Deoxyguanosine
Anti-Bacterial Agents pharmacology
Bacteriophage lambda chemistry
Bacteriophage lambda drug effects
Bacteriophage lambda radiation effects
Comet Assay
DNA Damage
DNA, Bacterial chemistry
DNA, Bacterial drug effects
DNA, Bacterial radiation effects
DNA, Viral chemistry
DNA, Viral drug effects
DNA, Viral radiation effects
Deoxyguanosine chemistry
Deoxyguanosine radiation effects
Oxidation-Reduction
Photochemistry
Staphylococcus aureus chemistry
Deoxyguanosine analogs & derivatives
Phenothiazines pharmacology
Photosensitizing Agents pharmacology
Staphylococcus aureus drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0928-8244
- Volume :
- 43
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- FEMS immunology and medical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 15708310
- Full Text :
- https://doi.org/10.1016/j.femsim.2004.09.002