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Efficient transformation of Cellulomonas flavigena by electroporation and conjugation with Bacillus thuringiensis.
- Source :
-
Current microbiology [Curr Microbiol] 2004 Dec; Vol. 49 (6), pp. 428-32. - Publication Year :
- 2004
-
Abstract
- The conjugative self-transmissible plasmid pHT73, harbored in Bacillus thuringiensis var. kurstaki, was demonstrated to be transferred to Cellulomonas flavigena, a cellulolytic bacterium. Both conjugation and transformation procedures yielded resistant colonies; however, chromosomal integration was observed only when bacterial conjugation occurred. The efficiency of conjugation was 10% of recipient strain, which is considered a very efficient process. When the plasmid pHT73 was introduced by transformation, erythromycin-resistant cells contained the plasmid as an episome with no arrangements, as assayed by Southern blot analysis. In contrast, conjugated-resistant cells harbor the plasmid integrated into the chromosome. These data suggest a common mechanism of cell communication between nonrelated bacterial species with similar ecological habitats, and also that both electroporation and conjugation can be used to transform C. flavigena efficiently.
Details
- Language :
- English
- ISSN :
- 0343-8651
- Volume :
- 49
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Current microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 15696619
- Full Text :
- https://doi.org/10.1007/s00284-004-4329-0