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An on-line technique for monitoring propionic acid fermentation.
- Source :
-
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2005 Aug; Vol. 68 (3), pp. 316-21. Date of Electronic Publication: 2005 Jan 29. - Publication Year :
- 2005
-
Abstract
- An on-line technique, based on measuring the increase in pressure due to CO(2) release in a closed air-tight reactor, was used to evaluate the fermentation of lactate by propionibacteria. The method was applied to batch cultures of Propionibacterium shermanii grown in yeast extract/sodium lactate medium containing lactate as a carbon source under micro-aerophilic conditions. Gas pressure evolution was compared both with substrate consumption and metabolites production and with acidification and growth. Linear relationships were found between gas pressure variation, lactate consumption and propionate and acetate production. The technique also enabled the evaluation of total CO(2) produced, by taking account of pressure, oxygen and pH measurements. These results tend to show that this simple and rapid method could be useful to monitor propionic acid bacteria growth.
- Subjects :
- Acetates metabolism
Bioreactors
Fermentation
Hydrogen-Ion Concentration
Lactic Acid metabolism
Manometry methods
Oxygen analysis
Oxygen metabolism
Pressure
Propionibacterium growth & development
Propionibacterium metabolism
Carbon Dioxide metabolism
Industrial Microbiology methods
Online Systems
Propionates metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0175-7598
- Volume :
- 68
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Applied microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 15682290
- Full Text :
- https://doi.org/10.1007/s00253-005-1888-3