Back to Search
Start Over
Antiviral treatment with alpha interferon up-regulates CD14 on liver macrophages and its soluble form in patients with chronic hepatitis B.
- Source :
-
Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2005 Feb; Vol. 49 (2), pp. 590-9. - Publication Year :
- 2005
-
Abstract
- To investigate whether therapy with alpha interferon (IFN-alpha) induces changes in intrahepatic antigen-presenting cells (APCs), we obtained liver biopsy specimens before, during, and after therapy with IFN-alpha from chronic hepatitis B patients whose viral load had already been reduced by at least 8 weeks of treatment with lamivudine. HLA-DR, CD1a, and CD83 were not modified by the therapy. The intralobular expression of CD68 on Kupffer cells remained stable, denoting no changes in the number of resident macrophages during IFN-alpha treatment. In contrast, CD14 was weakly expressed in the absence of IFN-alpha and was significantly up-regulated during therapy. At the same time, the levels of soluble CD14 and interleukin-10 in plasma increased significantly. In vitro, monocytes maintained in the presence of IFN-alpha differentiated into macrophages or dendritic cells with higher levels of expression of CD14 than that for the control cultures. During therapy with IFN-alpha, T-cell infiltration in the portal spaces was reduced, mainly due to a significant decrease in the number of CD8(+) T cells. These findings show that IFN-alpha is biologically active on APCs in vivo and in vitro and suggest that this newly described regulatory function, together with the already known inhibitory effects on lymphocytes, may cooperate to reduce inflammation and consequent tissue damage in patients with chronic viral hepatitis.
- Subjects :
- CD8-Positive T-Lymphocytes drug effects
Dendritic Cells drug effects
Hepatitis B, Chronic pathology
Hepatitis B, Chronic virology
Humans
Immunohistochemistry
Interleukin-10 blood
Lamivudine therapeutic use
Liver drug effects
Macrophages drug effects
Phenotype
Recombinant Proteins
Up-Regulation drug effects
Antiviral Agents therapeutic use
Hepatitis B, Chronic drug therapy
Interferon Type I therapeutic use
Lipopolysaccharide Receptors biosynthesis
Liver pathology
Macrophages metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0066-4804
- Volume :
- 49
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Antimicrobial agents and chemotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 15673738
- Full Text :
- https://doi.org/10.1128/AAC.49.2.590-599.2005