Back to Search
Start Over
Human umbilical cord perivascular (HUCPV) cells: a source of mesenchymal progenitors.
- Source :
-
Stem cells (Dayton, Ohio) [Stem Cells] 2005 Feb; Vol. 23 (2), pp. 220-9. - Publication Year :
- 2005
-
Abstract
- We describe the isolation of a nonhematopoietic (CD45-, CD34-, SH2+, SH3+, Thy-1+, CD44+) human umbilical cord perivascular (HUCPV) cell population. Each HUCPV cell harvest (2-5 x 10(6), depending on the length of cord available) gave rise to a morphologically homogeneous fibroblastic cell population, which expressed alpha-actin, desmin, vimentin, and 3G5 (a pericyte marker) in culture. We determined the colony-forming unit-fibro-blast (CFU-F) frequency of primary HUCPV cells to be 1:333 and the doubling time, which was 60 hours at passage 0 (P0), decreased to 20 hours at P2. This resulted in a significant cell expansion, producing over 10(10) HUCPV cells within 30 days of culture. Furthermore, HUCPV cells cultured in nonosteogenic conditions contained a subpopulation that exhibited a functional osteogenic phenotype and elaborated bone nodules. The frequency of this CFU-osteogenic subpopulation at P1 was 2.6/10(5) CFU-F, which increased to 7.5/10(5) CFU-F at P2. Addition of osteogenic supplements to the culture medium resulted in these frequencies increasing to 1.2/10(4) and 1.3/10(4) CFU-F, respectively, for P1 and P2. CFU-O were not seen at P0 in either osteogenic or non-osteogenic culture conditions, but P0 HUCPV cells did contain a 20% subpopulation that presented neither class I nor class II cell-surface major histocompatibility complexes (MHC-/-). This population increased to 95% following passage and cryopreservation (P5). We conclude that, due to their rapid doubling time, high frequencies of CFU-F and CFU-O, and high MHC-/- phenotype, HUCPV cells represent a significant source of cells for allogeneic mesenchymal cell-based therapies.
- Subjects :
- Cells, Cultured
Colony-Forming Units Assay
Fibroblasts cytology
Fibroblasts physiology
Humans
Mesenchymal Stem Cells cytology
Osteogenesis physiology
Umbilical Cord cytology
Umbilical Cord ultrastructure
Cell Differentiation physiology
Mesenchymal Stem Cells physiology
Umbilical Cord physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1066-5099
- Volume :
- 23
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Stem cells (Dayton, Ohio)
- Publication Type :
- Academic Journal
- Accession number :
- 15671145
- Full Text :
- https://doi.org/10.1634/stemcells.2004-0166