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GFAP immunoreactivity and transcription in trigeminal and dental tissues of rats and transgenic GFP/GFAP mice.
- Source :
-
Microscopy research and technique [Microsc Res Tech] 2004 Dec; Vol. 65 (6), pp. 295-307. - Publication Year :
- 2004
-
Abstract
- Sensory mechanisms in teeth are not well understood and may involve pulpal-neural interactions. Tooth cells that proliferate in vitro have polyclonal immunoreactivity (IR) for glial fibrillary acidic protein (GFAP), growth-associated protein (GAP-43), and vimentin, plus glial-like ion channels. Here, we analyzed GFAP-IR patterns in dental and trigeminal tissues of rats, for comparison with green fluorescent protein (GFP) associated with GFAP transcription in transgenic mice, in order to better characterize glial-like cells in dental tissues. Astrocytes, ganglion satellite cells, and epineurial Schwann cells were demonstrated by anti-GFAP antibodies and GFP-GFAP, as expected. Odontoblasts did not stain by any of these methods and cannot be the glial-like cells. Fibroblasts and undifferentiated mesenchymal cells in pulp had polyclonal GFAP-IR and vimentin-IR, while nerve fibers reacted only with polyclonal antibody. Some Schwann cell subtypes in trigeminal nerve and oral mucosa were positive for GFP and for polyclonal anti-GFAP, but not for monoclonal antibody. In pulp almost all Schwann cells were unstained, but many Schwann cells in periodontal ligament had polyclonal GFAP-IR. These results show greater heterogeneity for Schwann cells than expected, and suggest that the glial-like pulp cells are fibroblasts and/or undifferentiated mesenchymal cells or stem cells. We also found that polyclonal GFAP revealed intermediate filaments in preterminal sensory nerve fibers, thereby providing a useful marker for that neural subregion. GFP transcription by some Schwann cell subtypes in oral mucosae and trigeminal nerve, but not trigeminal root was a novel finding that reveals more complexity in peripheral glia than previously recognized.<br /> (Copyright 2005 Wiley-Liss, Inc.)
- Subjects :
- Animals
Female
Glial Fibrillary Acidic Protein genetics
Immunohistochemistry
Male
Mice
Mice, Transgenic
Mouth Mucosa chemistry
Odontoblasts chemistry
Rats
Rats, Sprague-Dawley
Transcription, Genetic
Vimentin analysis
Dental Pulp chemistry
Gingiva chemistry
Glial Fibrillary Acidic Protein analysis
Periodontal Ligament chemistry
Trigeminal Ganglion chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1059-910X
- Volume :
- 65
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Microscopy research and technique
- Publication Type :
- Academic Journal
- Accession number :
- 15662620
- Full Text :
- https://doi.org/10.1002/jemt.20130