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Four peptide hormones decrease the number of human breast adenocarcinoma cells.
- Source :
-
European journal of clinical investigation [Eur J Clin Invest] 2005 Jan; Vol. 35 (1), pp. 60-9. - Publication Year :
- 2005
-
Abstract
- Background: A family of six hormones, i.e. atrial natriuretic peptide, brain natriuretic peptide, C-natriuretic peptide, long-acting natriuretic peptide, vessel dilator, and kaliuretic peptide's main known biologic properties are sodium and water excreting and blood pressure lowering.<br />Methods and Materials: These six hormones, each at their 1-microm concentrations, were evaluated for their ability to decrease the number and/or proliferation of breast adenocarcinoma cells in culture for 24, 48, 72, and 96 h.<br />Results: Within 24 h, vessel dilator, long-acting natriuretic peptide, kaliuretic peptide, atrial natriuretic peptide and 8-bromo-cyclic GMP, a cell-permeable analogue of their intracellular mediator cyclic GMP (each at 1 microm), decreased the number of breast adenocarcinoma cells 60%, 31%, 27%, 40%, and 31%, respectively. There was no proliferation in the 3 days following this decrease in breast adenocarcinoma cell number. These same hormones decreased DNA synthesis 69% to 85% (P < 0.001). Brain natriuretic peptide and CNP did not decrease the number of breast adenocarcinoma cells or inhibit their DNA synthesis. Vessel dilator, long-acting natriuretic peptide, kaliuretic peptide and 8-bromo-cyclic GMP (each at 1 microM) decreased the number of cells in the S phase of the cell cycle by 62%, 33%, 50%, and 39%, respectively (all P < 0.05). Natriuretic peptide receptors-A and -C were present in the breast adenocarcinoma cells.<br />Conclusions: Four peptide hormones significantly decrease the number of human breast adenocarcinoma cells within 24 h and inhibit the proliferation of these cells for at least 96 h. Their mechanism of doing so involves inhibition of DNA synthesis and a decrease in cells in the S phase of the cell cycle mediated in part by cyclic GMP.
- Subjects :
- Adenocarcinoma metabolism
Adenocarcinoma pathology
Atrial Natriuretic Factor pharmacology
Blotting, Western methods
Breast Neoplasms metabolism
Breast Neoplasms pathology
Cell Cycle drug effects
Cell Line, Tumor
Cell Proliferation drug effects
Cyclic AMP metabolism
DNA biosynthesis
Female
Guanylate Cyclase analysis
Humans
Middle Aged
Natriuretic Peptide, Brain pharmacology
Natriuretic Peptide, C-Type pharmacology
Peptide Fragments pharmacology
Protein Precursors pharmacology
Receptors, Atrial Natriuretic Factor analysis
Adenocarcinoma drug therapy
Breast Neoplasms drug therapy
Hormones pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2972
- Volume :
- 35
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- European journal of clinical investigation
- Publication Type :
- Academic Journal
- Accession number :
- 15638821
- Full Text :
- https://doi.org/10.1111/j.1365-2362.2005.01444.x