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Molecular epidemiology of pneumococcal colonization in response to pneumococcal conjugate vaccination in children with recurrent acute otitis media.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2005 Jan; Vol. 43 (1), pp. 74-83. - Publication Year :
- 2005
-
Abstract
- A randomized double-blind trial with a 7-valent pneumococcal conjugate vaccine was conducted in The Netherlands among 383 children, aged 1 to 7 years, with a history of recurrent acute otitis media. No effect of vaccination on the pneumococcal colonization rate was found. However, a shift in serotype distribution was clearly observed (R. Veenhoven et al., Lancet 361:2189-2195, 2003). We investigated the molecular epidemiology of 921 pneumococcal isolates retrieved from both the pneumococcal vaccine (PV) and control vaccine (CV) groups during the vaccination study. Within individuals a high turnover rate of pneumococcal restriction fragment end labeling genotypes, which was unaffected by vaccination, was observed. Comparison of the genetic structures before and after completion of the vaccination scheme revealed that, despite a shift in serotypes, there was clustering of 70% of the pneumococcal populations. The remaining isolates (30%) were equally observed in the PV and CV groups. In addition, the degree of genetic clustering was unaffected by vaccination. However, within the population genetic structure, nonvaccine serotype clusters with the serotypes 11, 15, and 23B became predominant over vaccine-type clusters after vaccination. Finally, overall pneumococcal resistance was low (14%), and, albeit not significant, a reduction in pneumococcal resistance as a result of pneumococcal vaccination was observed. Molecular surveillance of colonization in Dutch children shows no effect of pneumococcal conjugate vaccination on the degree of genetic clustering and the genetic structure of the pneumococcal population. However, within the genetic pneumococcal population structure, a clear shift toward nonvaccine serotype clusters was observed.
- Subjects :
- Acute Disease
Child
Child, Preschool
Double-Blind Method
Humans
Infant
Molecular Epidemiology
Netherlands epidemiology
Otitis Media epidemiology
Otitis Media prevention & control
Pneumococcal Infections microbiology
Pneumococcal Infections prevention & control
Pneumococcal Vaccines therapeutic use
Recurrence
Streptococcus pneumoniae classification
Streptococcus pneumoniae isolation & purification
Vaccination
Vaccines, Conjugate therapeutic use
Nasopharynx microbiology
Otitis Media microbiology
Pneumococcal Vaccines administration & dosage
Streptococcus pneumoniae genetics
Streptococcus pneumoniae growth & development
Vaccines, Conjugate administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 0095-1137
- Volume :
- 43
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 15634953
- Full Text :
- https://doi.org/10.1128/JCM.43.1.74-83.2005