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Restricted IgA repertoire in both B-1 and B-2 cell-derived gut plasmablasts.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2005 Jan 15; Vol. 174 (2), pp. 1046-54. - Publication Year :
- 2005
-
Abstract
- Mucosal IgA is the most abundantly produced Ig upon colonization of the intestinal tract with commensal organisms in the majority of mammals. The repertoire of these IgA molecules is still largely unknown; a large amount of the mucosal IgA cannot be shown to react with the inducing microorganisms. Analysis of the repertoire of used H chain Ig (V(H)) genes by H-CDR3 spectrotyping, cloning, and sequencing of V(H) genes from murine intestinal IgA-producing plasma cells reveals a very restricted usage of V(H) genes and multiple clonally related sequences. The restricted usage of V(H) genes is a very consistent observation, and is observed for IgA plasma cells derived from B-1 or conventional B-2 cells from different mouse strains. Clonal patterns from all analyzed V(H) gene sequences show mainly independently acquired somatic mutations in contrast to the clonal evolution patterns often observed as a consequence of affinity maturation in germinal center reactions in peripheral lymphoid organs and Peyer's patches. Our data suggest a model of clonal expansion in which many mucosal IgA-producing B cells develop in the absence of affinity maturation. The affinity of most produced IgA might not be the most critical factor for its possible function to control the commensal organisms, but simply the abundance of large amounts of IgA that can bind with relatively unselected affinity to redundant epitopes on such organisms.
- Subjects :
- Animals
B-Lymphocyte Subsets immunology
B-Lymphocyte Subsets metabolism
Base Sequence
Cloning, Molecular
Immunoglobulin A genetics
Immunoglobulin Heavy Chains biosynthesis
Immunoglobulin Heavy Chains genetics
Immunoglobulin Heavy Chains isolation & purification
Immunoglobulin Variable Region biosynthesis
Immunoglobulin Variable Region genetics
Immunoglobulin Variable Region isolation & purification
Intestinal Mucosa cytology
Mice
Mice, Inbred A
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred CBA
Molecular Sequence Data
Peyer's Patches cytology
Peyer's Patches immunology
Peyer's Patches metabolism
Reverse Transcriptase Polymerase Chain Reaction
Somatic Hypermutation, Immunoglobulin
Species Specificity
Stem Cells immunology
Stem Cells metabolism
Immunoglobulin A biosynthesis
Intestinal Mucosa immunology
Intestinal Mucosa metabolism
Plasma Cells immunology
Plasma Cells metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 174
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 15634929
- Full Text :
- https://doi.org/10.4049/jimmunol.174.2.1046