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[Biochemical and physical properties for a recombinant IL6 Pseudomonas exotoxin fusion protein IL6D24-PE40KDEL].
- Source :
-
Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2004 Dec; Vol. 12 (6), pp. 825-8. - Publication Year :
- 2004
-
Abstract
- The objective was to identify some biochemical and physical properties for fusion protein IL6D24-PE40KDEL. Edman degradation, SDS-PAGE, peptide mass fingerprinting, Western blot and MTT were used for identification of the protein. The results showed that the sequence of N-terminus is Met-Ile-Asp-Lys-Gln-Ile, Met was added because of prokaryotic expression system; Western blot revealed that the purified protein could react with IL6 and PEA antibody. The purified protein IL6D24-PE40KDEL could kill the multiple myeloma cell lines U266 expressing high affinity IL6R, but it could not kill the cell lines CEM which not expressed IL6R; The molecular weight was 58.7 kD measuring by SDS-PAGE; peptide mass fingerprinting (PMF) confirmed that the construction of IL6D24-PE40KDEL was correct. A novel protein by Peptident database in EXPASY web site was identified. In conclusion, IL6D24-PE40KDEL is a new targeting protein with bioactivity of specific killing effect.
- Subjects :
- ADP Ribose Transferases chemistry
ADP Ribose Transferases pharmacology
Amino Acid Sequence
Blotting, Western
Cell Line, Tumor
Cell Survival drug effects
Dose-Response Relationship, Drug
Exotoxins chemistry
Exotoxins pharmacology
Humans
Interleukin-6 chemistry
Interleukin-6 pharmacology
Molecular Sequence Data
Pseudomonas aeruginosa genetics
Pseudomonas aeruginosa metabolism
Recombinant Fusion Proteins chemistry
Recombinant Fusion Proteins pharmacology
Sequence Analysis, Protein
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
ADP Ribose Transferases metabolism
Exotoxins metabolism
Interleukin-6 metabolism
Recombinant Fusion Proteins metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1009-2137
- Volume :
- 12
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Zhongguo shi yan xue ye xue za zhi
- Publication Type :
- Academic Journal
- Accession number :
- 15631670