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Oligonucleotide array for detection of common severe determinants of alpha thalassemia.
- Source :
-
Journal of biotechnology [J Biotechnol] 2005 Jan 12; Vol. 115 (1), pp. 1-9. - Publication Year :
- 2005
-
Abstract
- A simple and an efficient oligonucleotide array was developed to identify common severe determinants of alpha (alpha) thalassemia. A total of 14 probes were designed to detect the most frequently three deletions (-alpha(3.7), -alpha(4.2), -(SEA)) and two non-deletions (alpha(Quong Sze), alpha(Constant Spring)). PCR products were amplified from human genomic DNA and allowed to hybridize with the oligonucleotide array. Hybridization was detected by fluorescence scanning, and alpha globin genotypes were assigned by quantitative analysis of the hybridization results. The efficiency and specificity of identifying alpha globin genotypes using the oligonucleotide arrays was evaluated by blinded analysis of 690 samples from unrelated individuals. The oligonucleotide array method described in this paper provides unambiguous detection of complex combinations of heterozygous, compound heterozygous and homozygous alpha thalassemia genotypes. The experimental results demonstrate that this methodological approach may be applied for screening and for hemological diagnosis in population at large.
- Subjects :
- Biomarkers analysis
China epidemiology
DNA Mutational Analysis methods
Humans
Polymorphism, Genetic
Reproducibility of Results
Risk Factors
Sensitivity and Specificity
Severity of Illness Index
alpha-Thalassemia genetics
Genetic Predisposition to Disease epidemiology
Genetic Testing methods
Hemoglobin H genetics
Oligonucleotide Array Sequence Analysis methods
Risk Assessment methods
alpha-Thalassemia epidemiology
alpha-Thalassemia metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0168-1656
- Volume :
- 115
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 15607220
- Full Text :
- https://doi.org/10.1016/j.jbiotec.2004.07.016