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Impaired cognitive performance in neuronal nitric oxide synthase knockout mice is associated with hippocampal protein derangements.
- Source :
-
Nitric oxide : biology and chemistry [Nitric Oxide] 2004 Dec; Vol. 11 (4), pp. 316-30. Date of Electronic Publication: 2004 Nov 25. - Publication Year :
- 2004
-
Abstract
- Nitric oxide is implicated in modulation of memory and pharmacological as well as genetic inhibition of neuronal nitric oxide synthase (nNOS) leads to impaired cognitive function. We therefore decided to study learning and memory functions and cognitive flexibility in the Morris water maze (MWM) in 1-month-old male mice lacking nNOS (nNOS KO). Hippocampal protein profiling was carried out to possibly link protein derangement to impaired cognitive function. Two-dimensional gel electrophoresis with in-gel digestion of spots and subsequent MALDI-TOF identification of proteins and quantification of proteins using specific software was applied. In the memory as well as in the relearning task of the MWM, most of the nNOS KO failed to find the submerged platform within a given time. Proteomic evaluation of hippocampus, the main anatomical structure computing cognitive functions, revealed aberrant expression of a synaptosomal associated protein of the exocytotic machinery (NSF), glycolytic enzymes, chaperones 78 kDa glucose-regulated protein, T-complex protein 1; the signaling structure guanine nucleotide-binding protein G(I)/G(S)/G(T) and heterogeneous nuclear ribonucleoprotein H of the splicing machinery. We conclude that nNOS knockout mice show impaired spatial performance in the MWM, a finding that may be either linked to direct effects of nNOS/NO and/or to specific hippocampal protein derangements.
- Subjects :
- Animals
Hippocampus metabolism
Male
Mice
Mice, Knockout
Nerve Tissue Proteins genetics
Nitric Oxide physiology
Nitric Oxide Synthase genetics
Nitric Oxide Synthase Type I
Cognition physiology
Hippocampus physiology
Nerve Tissue Proteins metabolism
Nerve Tissue Proteins physiology
Nitric Oxide Synthase physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1089-8603
- Volume :
- 11
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Nitric oxide : biology and chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 15604044
- Full Text :
- https://doi.org/10.1016/j.niox.2004.10.005