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Gene expression profiles for detecting and distinguishing potential endocrine-disrupting compounds in environmental samples.
- Source :
-
Environmental science & technology [Environ Sci Technol] 2004 Dec 01; Vol. 38 (23), pp. 6396-406. - Publication Year :
- 2004
-
Abstract
- Industrial and municipal processes may produce and release endocrine-disrupting compounds (EDCs) into the environment, but the exact nature of their effects is difficult to investigate. EDCs typically exert their effect by affecting gene expression aberrantly. To determine if gene expression profiles could be used to detect and distinguish estrogenic EDCs, an estrogen receptor positive human breast cancer cell line (MCF-7) was exposed to known estrogenic compounds, suspected EDCs, and extracts from three effluent samples. A set of specifically estrogen-regulated genes was identified by microarray analysis. Nine estrogen up-regulated genes (IGFBP4, HSPA8, B4GALT1, XBP1, KRT8, GTPBP4, HNRPAB, SLC2A1, and CALM1) and two estrogen down-regulated genes (ID2 and ZNF217) were consistently detectable in response to estrogen and other estrogenic compounds. Gene expression patterns in cells that were exposed to effluent sample extracts were compared to gene expression patterns in cells that were exposed to known endocrines. Using this technique, two of the effluent samples were shown to have estrogenic activity. This approach could easily be extended to screen for other types of receptor-mediated endocrine disruption. For example, cells expressing androgen or aryl hydrocarbon receptors could be used in gene expression profiling assays to detect androgenic effects or for the presence of bioactive aromatic hydrocarbons. Gene expression profiling is emerging as a sensitive and specific method to screen complex samples for endocrine disrupting activity.
- Subjects :
- Androgens analysis
Cell Line, Tumor
Endocrine System metabolism
Environmental Pollutants analysis
Estrogens analysis
Gene Expression Regulation, Neoplastic physiology
Humans
Hydrocarbons, Aromatic analysis
Hydrocarbons, Aromatic toxicity
Oligonucleotide Array Sequence Analysis
Receptors, Androgen metabolism
Receptors, Estrogen metabolism
Endocrine System drug effects
Environmental Pollutants toxicity
Gene Expression Profiling
Gene Expression Regulation, Neoplastic drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0013-936X
- Volume :
- 38
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- Environmental science & technology
- Publication Type :
- Academic Journal
- Accession number :
- 15597897
- Full Text :
- https://doi.org/10.1021/es049235r