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Production of polyclonal antibodies in rabbits.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2005; Vol. 295, pp. 27-40. - Publication Year :
- 2005
-
Abstract
- The generation of polyclonal antibodies to an antigen of interest is an important technique applicable to many areas of biological research. In this chapter, we describe a basic immunization procedure designed to generate polyclonal antisera in rabbits and two methods that are commonly employed in the subsequent preliminary characterization of antipeptide antibodies raised in this way.
- Subjects :
- Animals
Rabbits
Antibodies
Immunization methods
Peptides
Subjects
Details
- Language :
- English
- ISSN :
- 1064-3745
- Volume :
- 295
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 15596886