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Shiga toxin 1 and ricin inhibit the repair of H2O2-induced DNA single strand breaks in cultured mammalian cells.
- Source :
-
DNA repair [DNA Repair (Amst)] 2005 Feb 03; Vol. 4 (2), pp. 271-7. - Publication Year :
- 2005
-
Abstract
- A growing body of evidence suggests that ribosome-inactivating proteins (RIPs) remove adenine moieties not only from rRNA, but also from DNA--an effect leading to DNA damage in cultured cells. We herein report that two distinct RIPs of bacterial (shiga toxin 1, Stx1) and plant (ricin) origin, inhibit the repair of the DNA lesions generated by hydrogen peroxide in cultured human cells. This effect is unrelated either to inhibition of protein synthesis or to depletion of cellular antioxidant defenses and is likely to derive from direct interactions with cellular DNA repair machinery. Therefore, the genotoxicity of these toxins on mammalian cells seems to be a complex phenomenon resulting from the balance between direct (DNA damaging activity), indirect (DNA repair inhibition) effects and the eventual presence of other DNA damaging species. In particular, with regard to Stx1, it could be hypothesized that Stx-producing bacteria increase the risk of transformation of surrounding, inflamed tissues in the course of human infections.
- Subjects :
- Apoptosis drug effects
Catalase metabolism
Cell Nucleus drug effects
Cells, Cultured
DNA genetics
DNA Damage
Endothelium, Vascular drug effects
Endothelium, Vascular metabolism
Glutathione Peroxidase metabolism
Humans
Oxidants pharmacology
Protein Biosynthesis drug effects
DNA metabolism
DNA Repair drug effects
DNA Repair genetics
Hydrogen Peroxide pharmacology
Ricin adverse effects
Shiga Toxin 1 adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 1568-7864
- Volume :
- 4
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- DNA repair
- Publication Type :
- Academic Journal
- Accession number :
- 15590335
- Full Text :
- https://doi.org/10.1016/j.dnarep.2004.09.007