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Rapid increase of bile salt secretion is associated with bile duct injury after human liver transplantation.
- Source :
-
Journal of hepatology [J Hepatol] 2004 Dec; Vol. 41 (6), pp. 1017-25. - Publication Year :
- 2004
-
Abstract
- Background/aims: Biliary strictures are a serious cause of morbidity after liver transplantation. We have studied the role of altered bile composition as a mechanism of bile duct injury after human liver transplantation.<br />Methods: In 28 liver transplant recipients, bile samples were collected daily posttransplantation for determination of bile composition. Hepatic expression of bile transporters was studied before and after transplantation. Histopathological criteria as well as biliary concentrations of alkaline phosphatase (ALP) and gamma-glutamyltransferase (gamma-GT) were used to quantify bile duct injury.<br />Results: Early after transplantation, bile salt secretion increased more rapidly than phospholipid secretion, resulting in high biliary bile salt/phospholipid ratio (BA/PL). In parallel with this, mRNA levels of the bile salt transporters NTCP and BSEP increased significantly after transplantation, whereas phospholipid translocator MDR3 mRNA levels remained unchanged. Bile duct injury correlated significantly with bile salt secretion and was associated with a high biliary BA/PL ratio.<br />Conclusions: Bile salt secretion after human liver transplantation recovers more rapidly than phospholipid secretion. This results in cytotoxic bile formation and correlates with bile duct injury. These findings suggest that endogenous bile salts have a role in the pathogenesis of bile duct injury after liver transplantation.
- Subjects :
- ATP Binding Cassette Transporter, Subfamily B genetics
ATP Binding Cassette Transporter, Subfamily B metabolism
ATP-Binding Cassette Transporters genetics
ATP-Binding Cassette Transporters metabolism
Bile metabolism
Fluorescent Antibody Technique
Humans
Membrane Transport Proteins genetics
Membrane Transport Proteins metabolism
Organic Anion Transporters, Sodium-Dependent genetics
Organic Anion Transporters, Sodium-Dependent metabolism
Phospholipids metabolism
Postoperative Period
RNA, Messenger metabolism
Symporters genetics
Symporters metabolism
Time Factors
Bile Acids and Salts metabolism
Bile Duct Diseases etiology
Liver metabolism
Liver Transplantation adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 0168-8278
- Volume :
- 41
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of hepatology
- Publication Type :
- Academic Journal
- Accession number :
- 15582136
- Full Text :
- https://doi.org/10.1016/j.jhep.2004.08.023