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Selective silencing of angiotensin receptor subtype 1a (AT1aR) by RNA interference.
- Source :
-
Hypertension (Dallas, Tex. : 1979) [Hypertension] 2005 Jan; Vol. 45 (1), pp. 115-9. Date of Electronic Publication: 2004 Nov 29. - Publication Year :
- 2005
-
Abstract
- Angiotensin II exerts its physiological effects by activating multiple subtypes of its receptor such as AT1a-, AT1b-, and AT2-receptors. Because of a high degree of similarity among these G-protein-coupled receptors, it has been difficult to assign diverse physiological actions of angiotensin II through these receptor subtypes. We have developed small interfering RNAs to selectively inhibit the expression of the AT1a receptor (AT1aR) subtype. A dsRNA, AT1 47, was found to be highly selective and efficient in reducing the levels of AT1aR subtype. Transfection of AT1aR-expressing CHO cells with dsRNA AT1 47 resulted in an 80% decrease in the AT1aR expression. In contrast, dsRNA AT1 47 showed no significant effects on both AT1bR and AT2R subtypes. Thus, AT1 47 provides us with a powerful tool to selectively silence this subtype of receptor to investigate its role in cardiovascular physiology.
- Subjects :
- 1-Sarcosine-8-Isoleucine Angiotensin II metabolism
Angiotensin II metabolism
Animals
CHO Cells
Calcium metabolism
Cricetinae
Cricetulus
Dose-Response Relationship, Drug
Ion Transport drug effects
Protein Binding
RNA, Messenger antagonists & inhibitors
Rats
Receptor, Angiotensin, Type 1 metabolism
Receptor, Angiotensin, Type 2 genetics
Receptor, Angiotensin, Type 2 metabolism
Recombinant Fusion Proteins biosynthesis
Recombinant Fusion Proteins physiology
Reverse Transcriptase Polymerase Chain Reaction
Transfection
Gene Silencing
RNA Interference
RNA, Double-Stranded pharmacology
RNA, Small Interfering pharmacology
Receptor, Angiotensin, Type 1 genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1524-4563
- Volume :
- 45
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Hypertension (Dallas, Tex. : 1979)
- Publication Type :
- Academic Journal
- Accession number :
- 15569855
- Full Text :
- https://doi.org/10.1161/01.HYP.0000150161.78556.c6