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BBE02 disruption mutants of Borrelia burgdorferi B31 have a highly transformable, infectious phenotype.
- Source :
-
Infection and immunity [Infect Immun] 2004 Dec; Vol. 72 (12), pp. 7147-54. - Publication Year :
- 2004
-
Abstract
- We constructed highly transformable and infectious Borrelia burgdorferi B31 by inactivating BBE02, a putative restriction-modification gene on the linear plasmid lp25. The low-passage-number B31 clones 5A4 (containing all plasmids) and 5A18 (lp28-4(-) lp56(-)) were used for this study, and BBE02 was disrupted by homologous recombination. The transformation efficiency with the shuttle vector pBSV2C03::gntDeltakan was increased from <1 to approximately 10 colonies per mug of DNA for 5A4 and 5A4 BBE02::Kan(r) and from 14 to approximately 600 colonies per mug of DNA for 5A18 and 5A18 BBE02::Kan(r). lp25, which is required for infectivity in mice, was retained in BBE02 mutants transformed with pBSV2C03::gntDeltakan, but lp25 was not detected in transformants of the parental clones 5A4 and 5A18. BBE02 disruptants and pBSV2C03::gntDeltakan transformants of these clones remained infectious in C3H/HeN mice, and the 50% infective doses of the BBE02 disruptants were <10(2) organisms per mouse. The inactivation of BBE02 thus eliminates a transformation barrier for infectious B. burgdorferi B31 and will provide a valuable tool for studying the virulence factors of Lyme disease.
- Subjects :
- Animals
Borrelia burgdorferi pathogenicity
Female
Joints pathology
Lyme Disease etiology
Lyme Disease pathology
Mice
Mice, Inbred C3H
Mutation
Nicotinamidase genetics
Phenotype
Transformation, Bacterial
Virulence Factors physiology
Bacterial Proteins physiology
Borrelia burgdorferi genetics
Nicotinamidase physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-9567
- Volume :
- 72
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 15557639
- Full Text :
- https://doi.org/10.1128/IAI.72.12.7147-7154.2004