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Human antibodies to recombinant protein constructs of Plasmodium falciparum Apical Membrane Antigen 1 (AMA1) and their associations with protection from malaria.
- Source :
-
Vaccine [Vaccine] 2004 Dec 16; Vol. 23 (5), pp. 718-28. - Publication Year :
- 2004
-
Abstract
- Serum antibodies from 1071 people in two Kenyan villages were assayed using eight different recombinant Apical Membrane Antigen 1 (AMA1) protein constructs to investigate their role in naturally acquired immunity. In both communities, antibodies against the full-length ectodomain (both FVO and 3D7 allele constructs) prior to a malaria transmission season were significantly associated with protection from malaria in the following 6 months, even after adjusting for age and antibody reactivity to whole parasite (schizont) extract. However, these protective associations of antibodies were only seen among subjects that were parasite slide positive at the time of pre-season serum sampling. Competition ELISAs with the FVO and 3D7 allele constructs showed that antibodies can recognise either conserved or allele-specific epitopes in AMA1. Results encourage the development of an AMA1 vaccine based on the full-length ectodomain, and indicate that the function of human antibodies to allele-specific and conserved epitopes in AMA1 should be studied further.
- Subjects :
- Adolescent
Adult
Aged
Animals
Antigens, Protozoan genetics
Antigens, Protozoan isolation & purification
Child
Child, Preschool
Cohort Studies
Enzyme-Linked Immunosorbent Assay
Epitopes immunology
Humans
Infant
Infant, Newborn
Kenya
Malaria Vaccines
Malaria, Falciparum prevention & control
Membrane Proteins genetics
Membrane Proteins isolation & purification
Middle Aged
Plasmodium falciparum genetics
Protozoan Proteins genetics
Protozoan Proteins isolation & purification
Seroepidemiologic Studies
Antibodies, Protozoan blood
Antigens, Protozoan immunology
Malaria, Falciparum immunology
Membrane Proteins immunology
Plasmodium falciparum immunology
Protozoan Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0264-410X
- Volume :
- 23
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 15542195
- Full Text :
- https://doi.org/10.1016/j.vaccine.2004.05.031