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Analysis of two alleles of the urease gene from potato: polymorphisms, expression, and extensive alternative splicing of the corresponding mRNA.
- Source :
-
Journal of experimental botany [J Exp Bot] 2005 Jan; Vol. 56 (409), pp. 91-9. Date of Electronic Publication: 2004 Nov 08. - Publication Year :
- 2005
-
Abstract
- Globally, urea is the most widely used nitrogen fertilizer and is made accessible to plants via the urease reaction. However, sequence information for the plant enzyme is scarce. A cDNA encoding urease from soybean (Glycine max) has been cloned and sequence information has been obtained for two alleles (11 and 19 kbp, respectively) of the potato (Solanum tuberosum, cv. Desiree) urease gene and the corresponding cDNAs. It was found that urease is encoded by a single copy gene in several solanaceous species, and maps to chromosome V in potato. By contrast, the presence of two urease genes was reported for soybean. Comparative analysis of 11 kbp overlapping allelic DNA allowed the quantification of single nucleotide polymorphisms and revealed the presence of a truncated Ty1-copia retrotransposon in one of the alleles. Both alleles contained a copy of a terminal-repeat retrotransposon in miniature (TRIM). 25-50% of urease pre-mRNAs from both alleles were alternatively spliced in a variety of different ways. The retrotransposons had no effect on splicing. While urease is expressed in all tissues tested, its mRNA and protein are of low abundance. The TATA-less urease promoter appears to drive low-level housekeeping transcription. An in silico analysis showed that eukaryotic urease protein sequences are very similar to sequences from prokaryotic enzymes, conserving all residues of known functional importance. It is therefore likely that all known ureases are structurally similar, employing the same catalytic mechanism.
- Subjects :
- Alternative Splicing
Amino Acid Sequence
Base Sequence
Conserved Sequence
Gene Expression
Molecular Sequence Data
Promoter Regions, Genetic
Protein Biosynthesis
RNA, Messenger metabolism
RNA, Plant metabolism
Sequence Alignment
Sequence Homology, Nucleic Acid
Species Specificity
Alleles
Polymorphism, Genetic genetics
Solanum tuberosum genetics
Urease genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0022-0957
- Volume :
- 56
- Issue :
- 409
- Database :
- MEDLINE
- Journal :
- Journal of experimental botany
- Publication Type :
- Academic Journal
- Accession number :
- 15533883
- Full Text :
- https://doi.org/10.1093/jxb/eri014