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Abnormal RNA processing and altered expression of serin-rich proteins in minimal-change nephrotic syndrome.
- Source :
-
Pediatric research [Pediatr Res] 2005 Jan; Vol. 57 (1), pp. 133-7. Date of Electronic Publication: 2004 Nov 05. - Publication Year :
- 2005
-
Abstract
- Mechanisms underlying the pathophysiology of minimal-change nephrotic syndrome (MCNS), the most frequent glomerular disease in children, remain elusive, but recent findings argue for a T cell dysfunction. Starting from a differential cDNA library from T cells of a patient under relapse and remission, we identified 16 transcripts specific for MCNS. All of these transcripts that were selectively up-regulated during the relapse phase of the disease were generated by alternative splicing of known genes. This abnormal RNA expression was associated with a down-regulation of serin-rich protein 75 and serin-rich protein 40, two proteins involved in mRNA splicing. Taken together, these data suggest that T cell dysfunction in MCNS is associated with abnormal mRNA splicing.
- Subjects :
- Child
Cohort Studies
DNA Primers chemistry
DNA, Complementary metabolism
Down-Regulation
Gene Library
Humans
Immunohistochemistry
Nephrotic Syndrome genetics
Phosphorylation
RNA metabolism
RNA Processing, Post-Transcriptional
RNA, Messenger metabolism
Recurrence
Remission Induction
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
T-Lymphocytes cytology
Up-Regulation
Alternative Splicing
Nephrotic Syndrome metabolism
Serine metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0031-3998
- Volume :
- 57
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Pediatric research
- Publication Type :
- Academic Journal
- Accession number :
- 15531747
- Full Text :
- https://doi.org/10.1203/01.PDR.0000148013.53429.5B