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Detection and selection of microsatellites in the genome of Paracoccidioides brasiliensis as molecular markers for clinical and epidemiological studies.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2004 Nov; Vol. 42 (11), pp. 5007-14. - Publication Year :
- 2004
-
Abstract
- Paracoccidioides brasiliensis, a thermodimorphic fungus, is the causative agent of the prevalent systemic mycosis in Latin America, paracoccidioidomycosis (PCM). Here, we describe the microsatellite patterns observed in a collection of P. brasiliensis random sequence tags. We identified 1,117 microsatellite patterns in about 3.8 Mb of unique sequences (0.47% of the total DNA used in the analysis). The majority of these microsatellites (87.5%) are found in noncoding sequences. We used two polymorphic microsatellites located on noncoding and coding sequences, as well as two microsatellites located on introns, as molecular markers to discriminate P. brasiliensis isolates, to look for relationships between the genetic background of the strains and the types of human disease they cause. We did not observe any correlation between the clinical form of human PCM and four simple sequence repeat patterns analyzed.
- Subjects :
- Animals
Armadillos microbiology
Genome, Fungal
Humans
Paracoccidioides genetics
Paracoccidioidomycosis epidemiology
Paracoccidioidomycosis microbiology
Phylogeny
Polymerase Chain Reaction
Virulence
Genetic Markers
Microsatellite Repeats genetics
Paracoccidioides classification
Paracoccidioides pathogenicity
Paracoccidioidomycosis physiopathology
Subjects
Details
- Language :
- English
- ISSN :
- 0095-1137
- Volume :
- 42
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 15528688
- Full Text :
- https://doi.org/10.1128/JCM.42.11.5007-5014.2004