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Detection and selection of microsatellites in the genome of Paracoccidioides brasiliensis as molecular markers for clinical and epidemiological studies.

Authors :
Nascimento E
Martinez R
Lopes AR
de Souza Bernardes LA
Barco CP
Goldman MH
Taylor JW
McEwen JG
Nobrega MP
Nobrega FG
Goldman GH
Source :
Journal of clinical microbiology [J Clin Microbiol] 2004 Nov; Vol. 42 (11), pp. 5007-14.
Publication Year :
2004

Abstract

Paracoccidioides brasiliensis, a thermodimorphic fungus, is the causative agent of the prevalent systemic mycosis in Latin America, paracoccidioidomycosis (PCM). Here, we describe the microsatellite patterns observed in a collection of P. brasiliensis random sequence tags. We identified 1,117 microsatellite patterns in about 3.8 Mb of unique sequences (0.47% of the total DNA used in the analysis). The majority of these microsatellites (87.5%) are found in noncoding sequences. We used two polymorphic microsatellites located on noncoding and coding sequences, as well as two microsatellites located on introns, as molecular markers to discriminate P. brasiliensis isolates, to look for relationships between the genetic background of the strains and the types of human disease they cause. We did not observe any correlation between the clinical form of human PCM and four simple sequence repeat patterns analyzed.

Details

Language :
English
ISSN :
0095-1137
Volume :
42
Issue :
11
Database :
MEDLINE
Journal :
Journal of clinical microbiology
Publication Type :
Academic Journal
Accession number :
15528688
Full Text :
https://doi.org/10.1128/JCM.42.11.5007-5014.2004