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Opposing functions of calcineurin and CaMKII regulate G-protein signaling in egg-laying behavior of C.elegans.
- Source :
-
Journal of molecular biology [J Mol Biol] 2004 Nov 19; Vol. 344 (2), pp. 585-95. - Publication Year :
- 2004
-
Abstract
- Ca(2+)/calmodulin-dependent calcineurin has been shown to have important roles in various Ca(2+) signaling pathways. We have previously reported that cnb-1(jh103) mutants, null mutants of a regulatory B subunit, displayed pleiotropic defects including uncoordinated movement and delayed egg laying in Caenorhabditis elegans. Interestingly, gain-of-function mutants of a catalytic A subunit showed exactly opposite phenotypes to those of cnb-1(null) mutants providing an excellent genetic model to define calcium-mediated signaling pathway at the organism level. Furthermore, calcineurin is also important for normal cuticle formation, which is required for maintenance of normal body size in C.elegans. Genetic interactions between tax-6 and several mutants including egl-30 and egl-10, which are known to be involved in G-protein signaling pathways suggest that calcineurin indeed regulates locomotion and serotonin-mediated egg laying through goa-1(Goalpha) and egl-30(Gqalpha). Our results indicate that, along with CaMKII, calcineurin regulates G-protein-coupled phosphorylation signaling pathways in C.elegans.
- Subjects :
- Animals
Body Size genetics
Caenorhabditis elegans drug effects
Caenorhabditis elegans ultrastructure
Caenorhabditis elegans Proteins
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Female
Gene Deletion
Green Fluorescent Proteins metabolism
Locomotion genetics
Models, Genetic
Phosphorylation
Recombinant Fusion Proteins metabolism
Reverse Transcriptase Polymerase Chain Reaction
Serotonin pharmacology
Signal Transduction
Two-Hybrid System Techniques
Caenorhabditis elegans metabolism
Calcineurin genetics
Calcineurin metabolism
Calcium-Calmodulin-Dependent Protein Kinases metabolism
GTP-Binding Proteins metabolism
Gene Expression Regulation, Developmental
Oviposition genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0022-2836
- Volume :
- 344
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 15522306
- Full Text :
- https://doi.org/10.1016/j.jmb.2004.09.050