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[Detection and sequence analysis of Sapporo-like virus in Guangzhou].

Authors :
Liu Y
Dai YC
Li JD
Zhan HC
Chen Q
Yu SY
Nie J
Source :
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA [Di Yi Jun Yi Da Xue Xue Bao] 2004 Oct; Vol. 24 (10), pp. 1147-9, 1152.
Publication Year :
2004

Abstract

Objective: To verify the presence of Sapporo-like virus (SLV) in children with diarrhea in Guangzhou.<br />Methods: Stool specimens were collected from children with diarrhea treated in the Department of Pediatrics of Nanfang Hospital in autumn and winter, 2003, and semi-nested reverse transcriptase-polymerase chain reaction (RT-PCR) was employed for SLV detection, the positive product of which was sequenced and analyzed in comparison with the SLV sequences documented in GenBank.<br />Results: Only one positive specimen (CH03354) was found out of the 169 stool specimens collected. Sequence analysis of the capsid gene of this strain showed that it belonged to SLV GI-1 cluster.<br />Conclusion: SLV exists in Guangzhou and different regions of China may have different genotypes of this virus.

Details

Language :
Chinese
ISSN :
1000-2588
Volume :
24
Issue :
10
Database :
MEDLINE
Journal :
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
Publication Type :
Academic Journal
Accession number :
15501736