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Suppression of 8-oxo-2'-deoxyguanosine formation and carcinogenesis induced by N-nitrosobis (2-oxopropyl)amine in hamsters by esculetin and esculin.
- Source :
-
Free radical research [Free Radic Res] 2004 Aug; Vol. 38 (8), pp. 839-46. - Publication Year :
- 2004
-
Abstract
- Effects of esculetin (6,7-dihydroxycoumarin) and its glycoside, esculin, on 8-oxo-2'-deoxyguanosine (8-oxodG) formation and carcinogenesis induced by a chemical carcinogen, N-nitrosobis(2-oxopropyl)amine (BOP), were examined in the pancreas of female Syrian golden hamsters. Animals were administered esculetin by gastric intubation into the stomach 30 min before BOP administration or ingestion of a diet containing esculin for 7 days before BOP administration, and killed 1 or 4h after BOP treatment, and the contents of thiobarbituric acid-reacting substrates (TBARS) and 8-oxodG in the pancreas were determined. Both compounds suppressed significantly the BOP-induced increases in 8-oxodG and TBARS contents in hamster pancreas. We further investigated the effect of esculin on pancreatic carcinogenesis by the rapid production model induced by augmentation pressure with a choline-deficient diet, ethionine, methionine and BOP. Esculin was given ad libitum as a 0.05% aqueous solution in either the initiation or promotion phases. The incidence of invasive tumors in animals given esculin during the initiation phase was significantly smaller than in the control group, while esculin given during the promotion phase showed no apparent effects. These results suggest that the intake of esculin has an inhibitory effect on BOP-induced oxidative DNA damage and carcinogenesis in hamster pancreas.
- Subjects :
- 8-Hydroxy-2'-Deoxyguanosine
Animal Feed
Animals
Body Weight drug effects
Carcinogens administration & dosage
Carcinogens pharmacology
Cell Nucleus drug effects
Cell Nucleus metabolism
Choline pharmacology
Cricetinae
DNA metabolism
Ethionine pharmacology
Female
Methionine pharmacology
Nitrosamines administration & dosage
Nitrosamines pharmacology
Organ Size drug effects
Oxidation-Reduction drug effects
Pancreas drug effects
Pancreas metabolism
Thiobarbituric Acid Reactive Substances metabolism
Carcinogens antagonists & inhibitors
Deoxyguanosine analogs & derivatives
Deoxyguanosine metabolism
Esculin pharmacology
Nitrosamines antagonists & inhibitors
Umbelliferones pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1071-5762
- Volume :
- 38
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Free radical research
- Publication Type :
- Academic Journal
- Accession number :
- 15493457
- Full Text :
- https://doi.org/10.1080/10715760410001715167