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WAVE1, an A-kinase anchoring protein, during mammalian spermatogenesis.
- Source :
-
Human reproduction (Oxford, England) [Hum Reprod] 2004 Nov; Vol. 19 (11), pp. 2594-604. Date of Electronic Publication: 2004 Oct 07. - Publication Year :
- 2004
-
Abstract
- Background: Proper compartmentalization of signalling cascades is paramount to many intracellular activities during spermatogenesis and sperm function. In the present study we focus on the A-kinase-anchoring protein (AKAP) WAVE1, a member of the Wiskott-Aldrich syndrome (WASP) family of adaptor proteins, to study its localization throughout mammalian spermatogenesis.<br />Methods: Using transmission electron microscopy, immunocytochemistry and western blotting, we examined the distribution of WAVE1 and putative partners during mammalian spermatogenesis. The localization and association of PKA RII, the regulatory subunit II of protein kinase A, tyrosine kinase Abl, and small GTPase RAC1 were also explored.<br />Results: WAVE1 localization in spermatocytes and round spermatids coincided with Golgi apparatus distribution, whereas in elongated spermatids and testicular sperm WAVE1 localized to the mitochondrial sheath. Following epididymal passage, WAVE1 was found exclusively on the mitochondrial sheath, suggesting that the protein may function in this region. WAVE1 and PKA RII co-localized along the mitochondrial sheath, PKA RII concentrates in the mid-piece, and RAC1 associated with the post-acrosomal region and the connecting piece. The distribution of WAVE1, PKA RII and RAC1 is conserved in mature mouse, bull, baboon and human sperm.<br />Conclusions: The data support the possibility of a functional signalling unit established by WAVE1 and its associated proteins in the mid-piece of maturing sperm.
- Subjects :
- Animals
Cattle
Cell Compartmentation
Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit
Epididymis cytology
Epididymis physiology
Golgi Apparatus metabolism
Humans
Male
Mice
Papio
Proteins metabolism
Spermatozoa metabolism
Wiskott-Aldrich Syndrome Protein Family
rac1 GTP-Binding Protein metabolism
Cyclic AMP-Dependent Protein Kinases metabolism
Mammals physiology
Microfilament Proteins metabolism
Spermatogenesis physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0268-1161
- Volume :
- 19
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Human reproduction (Oxford, England)
- Publication Type :
- Academic Journal
- Accession number :
- 15471936
- Full Text :
- https://doi.org/10.1093/humrep/deh513