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Phenotype and function of human natural killer cells purified by using a clinical-scale immunomagnetic method.
- Source :
-
Cancer immunology, immunotherapy : CII [Cancer Immunol Immunother] 2005 Apr; Vol. 54 (4), pp. 389-94. Date of Electronic Publication: 2004 Sep 22. - Publication Year :
- 2005
-
Abstract
- Infection, disease relapse, graft failure, and graft-versus-host disease (GVHD) are significant adverse events associated with allogeneic bone marrow transplantation. Donor natural killer (NK) cells may be an ideal cell type for prevention or treatment of all these adverse events. Therefore, we investigated the phenotype and function of human NK cells purified by using a clinical-scale immunomagnetic method. We found that the NK cell purification procedures did not adversely affect the expression of killer cell immunoglobulin-like receptors, adhesion molecules, intracellular cytokines, perforin, and granzyme B. Purified NK cells had extensive proliferative capacity and potent antitumor activity when assessed using an immunodeficient mouse model. While all mice transplanted with unpurified mononuclear cells developed GVHD, none of the mice transplanted with purified NK cells did. NK cells were highly susceptible to lysis by antithymocyte globulin (ATG), whereas G-CSF had a minimal effect on their natural cytotoxicity. These results support future clinical investigation of the use of purified NK cells for adoptive immunotherapy in the absence of ATG.
- Subjects :
- Animals
Antilymphocyte Serum pharmacology
Cell Adhesion Molecules metabolism
Cell Proliferation
Cytokines metabolism
Graft vs Host Disease immunology
Granulocyte Colony-Stimulating Factor pharmacology
Granzymes
Humans
Immunomagnetic Separation
Membrane Glycoproteins metabolism
Mice
Mice, Inbred NOD
Mice, SCID
Perforin
Pore Forming Cytotoxic Proteins
Receptors, Immunologic immunology
Serine Endopeptidases metabolism
Immunophenotyping
Killer Cells, Natural immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0340-7004
- Volume :
- 54
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Cancer immunology, immunotherapy : CII
- Publication Type :
- Academic Journal
- Accession number :
- 15449041
- Full Text :
- https://doi.org/10.1007/s00262-004-0609-6