Back to Search
Start Over
[Identification of two new strains of norovirus in Guangzhou].
- Source :
-
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA [Di Yi Jun Yi Da Xue Xue Bao] 2004 Sep; Vol. 24 (9), pp. 1016-8, 1022. - Publication Year :
- 2004
-
Abstract
- Objective: To identify the molecular biological characteristics of human caliciviruses (HuCVs) and new strains of norovirus (NV) detected in Guangdong Province.<br />Methods: HuCVs were detected from the stool specimens by reverse transcription-PCR (RT-PCR), and the PCR products were cloned, transformed, and blasted with Genbank. The nucleotide sequence homology was also evaluated with PHYLIP package, and the phylogenetic tree was generated by Treeview.<br />Results: HuCVs were detected from 18 out of 206 stool specimens from children with viral diarrhea (8.74%), which were all confirmed subsequently as NVs. Two strains HuCV/NVGII 003/2003/CHN(32282) and HuCV/NVGII 004/2003/CHN(32283) from the selected 6 NVs strain were identified as new NV strains.<br />Conclusion: New NV strains in consistency with the blood-type distribution of Chinese are present in Guangzhou, which have not been reported in other countries.
- Subjects :
- Base Sequence
Child, Preschool
China
DNA, Viral analysis
Female
Humans
Infant
Male
Molecular Sequence Data
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Caliciviridae Infections virology
Diarrhea, Infantile virology
Norovirus classification
Norovirus isolation & purification
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1000-2588
- Volume :
- 24
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
- Publication Type :
- Academic Journal
- Accession number :
- 15447850